Involvement of MMPs in the outward remodeling of collateral mesenteric arteries
| Autor: | Kevin M. Sheridan, Laura E. Norton, Joseph L. Unthank, Tara L. Haas, Matthew R. Distasi, Jennifer L. Doyle |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Time Factors Proto-Oncogene Proteins c-jun Physiology Collateral Circulation Matrix metalloproteinase Extracellular matrix Downregulation and upregulation Ileum Physiology (medical) Matrix Metalloproteinase 14 medicine Animals Protease Inhibitors Splanchnic Circulation Rats Wistar Ligation Mesenteric arteries Cell Proliferation Early Growth Response Protein 1 Chemistry Arteries Anatomy Collateral circulation Matrix Metalloproteinases Extracellular Matrix Mesenteric Arteries Rats Up-Regulation Cell biology Enzyme Activation Endothelial stem cell medicine.anatomical_structure Matrix Metalloproteinase 9 Doxycycline Models Animal Circulatory system Matrix Metalloproteinase 2 Stress Mechanical Tunica Intima Cardiology and Cardiovascular Medicine Immunostaining |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 293:H2429-H2437 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00100.2007 |
| Popis: | Persistent elevation in shear stress within conduit or resistance arteries causes structural luminal expansion, which serves to normalize shear stress while maintaining increased flow to the downstream vasculature. Although it is known that this adaptation involves cellular proliferation and remodeling of the extracellular matrix, the specific cellular events underlying these responses are poorly understood. Matrix metalloproteinases (MMPs) contribute to extensive remodeling of the extracellular matrix in conduit vessels and vein grafts exposed to high flow. However, involvement of MMPs in remodeling of small muscular collateral arteries, which are exposed to less severe increases in shear stress, has not been tested. We utilized an established model of outward remodeling in mesenteric collateral arteries to determine whether MMPs were upregulated during the remodeling response and to test whether MMP activity was required for luminal expansion. By 4 days, MMP-2 and membrane type 1 MMP (MT1-MMP), but not MMP-9, protein levels were significantly elevated in collateral arteries, as assessed by gelatin zymography and immunostaining. MMP-2 and MT1-MMP proteins, together with their respective transcriptional activators c-Jun and Egr-1 were localized predominantly to the smooth muscle layer of the collateral arteries. The general MMP inhibitor doxycycline prevented luminal expansion of collateral arteries but did not affect the endothelial cell proliferative or medial growth responses. In conclusion, this study provides evidence that MMP-2 and MT1-MMP are upregulated in collateral arteries exposed to elevated shear stress and that MMP activity is essential for the full remodeling response that leads to outward luminal expansion. |
| Databáze: | OpenAIRE |
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