Cost-Effective Application of Pulsed-Field Gel Electrophoresis to Typing of Salmonella enterica Serovar Typhimurium
| Autor: | G. Corbett-Feeney, Martin Cormican, Colette O'Hare, Geraldine Doran, Dearbháile Morris, Niall DeLappe, Bernard Bradshaw |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Serotype
Salmonella typhimurium viruses united-states netherlands serotype typhimurium Applied Microbiology and Biotechnology Microbiology Pulsed-field gel electrophoresis Animals Humans Typing Phage typing Gel electrophoresis outbreak Ecology biology pigs biochemical phenomena metabolism and nutrition biology.organism_classification bacterial infections and mycoses Virology Enterobacteriaceae Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Phage types cattle Salmonella enterica Food Microbiology chickens Salmonella Phages Food Science Biotechnology |
| Popis: | Salmonella enterica serovar Typhimurium is frequently isolated from humans and animals. Phage typing is historically the first-line reference typing technique in Europe. It is rapid and convenient for laboratories with appropriate training and experience, and costs of consumables are low. Phage typing and pulsed-field gel electrophoresis (PFGE) were performed on 503 isolates of serovar Typhimurium. Twenty-nine phage types and 53 PFGE patterns were observed. Most isolates of phage types DT104, DT104b, and U310 are not distinguishable from other members of their phage type by PFGE. By contrast, PFGE of isolates of phage types DT193 and U302 shows great heterogeneity. Analysis of experience with PFGE and phage typing can facilitate the selective application of PFGE to maximize the yield of epidemiologically relevant additional information while controlling costs. |
| Databáze: | OpenAIRE |
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