Characterization of a novel LFRFamide neuropeptide in the cephalopod Sepia officinalis

Autor: Jérôme Leprince, Hubert Vaudry, Céline Zatylny-Gaudin, Joël Henry, Benoit Bernay, Bruno Zanuttini
Přispěvatelé: Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Laboratoire de Physiogénomique / NeuroTranscriptomes et Paléogénétique (LPG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Equipe MAD - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU), Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), Centre de recherche en gestion (CRG), Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2010
Předmět:
Central Nervous System
Male
Cuttlefish
Sepia
Physiology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Amino Acid Sequence
MESH: Muscle
Smooth
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
Oviducts
MESH: Neuropeptides
Biochemistry
0302 clinical medicine
Endocrinology
MESH: Rectum
MESH: FMRFamide
Tandem Mass Spectrometry
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Hemolymph
MESH: Animals
Peptide sequence
Nerve Endings
0303 health sciences
MESH: Sepia
MESH: Nerve Endings
Muscle Tonus
MESH: Oligopeptides
Officinalis
Female
MESH: Animal Structures
Oligopeptides
Spectrometry
Mass
Electrospray Ionization
medicine.medical_specialty
Neuropeptide
Biology
MESH: Spectrometry
Mass
Electrospray Ionization
03 medical and health sciences
Cellular and Molecular Neuroscience
Internal medicine
medicine
MESH: Central Nervous System
Animals
Amino Acid Sequence
FMRFamide
030304 developmental biology
Neuropeptides
Rectum
MESH: Tandem Mass Spectrometry
Animal Structures
Muscle
Smooth
MESH: Muscle Tonus
biology.organism_classification
MESH: Male
Cephalopod
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
MESH: Female
MESH: Oviducts
030217 neurology & neurosurgery
Zdroj: Peptides
Peptides, Elsevier, 2010, 31 (2), pp.207-214. ⟨10.1016/j.peptides.2009.11.021⟩
Peptides, 2010, 31 (2), pp.207-214. ⟨10.1016/j.peptides.2009.11.021⟩
ISSN: 0196-9781
1873-5169
Popis: International audience; From a single LC-MS/MS analysis, a new C-terminally extended RFamide neuropeptide was characterized in Sepia officinalis. The experimental strategy was based on the specific neutral loss associated with RFamide breakdown. Mass losses of 17 Da (C-terminally amide) and 320 Da (RFamide) have been observed for three known peaks of m/z 581.7 (FLRFamide), 599.8 (FMRFamide), 1096.3 (ALSGDAFLRFamide) and one unknown of m/z 752.8. The primary sequence of the peptide of m/z 752.8 was GNLFRFamide. MS/MS analyses revealed that this novel neuropeptide, called sepFRF1, is largely distributed in the central nervous system of cuttlefish of both sexes. Probably transported in the visceral nerve from the subesophageal mass (the peptide was not detected in the hemolymph), this neuropeptide targeted the rectum in agreement with its peripheral distribution. From concentrations as low as 10(-9)M, sepFRF1 increased the frequency, tonus and amplitude of rectal contractions. SepFRF1 is the first RFamide peptide identified in Sepia officinalis that is not derived from the FaRPs precursor. SepFRF1 could belong to a RFamide subfamily identified in gastropods and may be involved in feeding behavior.
Databáze: OpenAIRE
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