Study of Long-Distance Functional Interactions between Su(Hw) Insulators That Can Regulate Enhancer-Promoter Communication in Drosophila melanogaster
| Autor: | Larisa Melnikova, Ekaterina Savitskaya, D. A. Chetverina, Ekaterina Pomerantseva, Polyna Zobacheva, Aleksander Parshikov, E. M. Gracheva, Elena Kravchenko, Alexander V. Galkin, Pavel Georgiev, Tatiana Boikova, Margarita Kostuchenko |
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| Rok vydání: | 2006 |
| Předmět: |
Genetics
Regulation of gene expression Retroelements biology Promoter Insulator (electricity) Retrotransposon Articles Cell Biology Computational biology biology.organism_classification Drosophila melanogaster Enhancer Elements Genetic Gene Expression Regulation Pairing Animals Insulator Elements Promoter Regions Genetic Enhancer Molecular Biology Insulator Element |
| Zdroj: | Molecular and Cellular Biology. 26:754-761 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.26.3.754-761.2006 |
| Popis: | The Su(Hw) insulator found in the gypsy retrotransposon is the most potent enhancer blocker in Drosophila melanogaster. However, two such insulators in tandem do not prevent enhancer-promoter communication, apparently because of their pairing interaction that results in mutual neutralization. Furthering our studies of the role of insulators in the control of gene expression, here we present a functional analysis of a large set of transgenic constructs with various arrangements of regulatory elements, including two or three insulators. We demonstrate that their interplay can have quite different outcomes depending on the order of and distance between elements. Thus, insulators can interact with each other over considerable distances, across interposed enhancers or promoters and coding sequences, whereby enhancer blocking may be attenuated, cancelled, or restored. Some inferences concerning the possible modes of insulator action are made from collating the new data and the relevant literature, with tentative schemes illustrating the regulatory situations in particular model constructs. |
| Databáze: | OpenAIRE |
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