Nanosilver Targets the Bacterial Cell Envelope: The Link with Generation of Reactive Oxygen Radicals
| Autor: | Riti Mann, Cindy Gunawan, Merisa Bestari Faiz, Rose Amal, S. R. Simon Ting, Georgios A. Sotiriou, Christopher P. Marquis |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Silver Materials science Metal Nanoparticles Peptidoglycan 02 engineering and technology 010402 general chemistry 01 natural sciences Bacterial cell structure Cell wall chemistry.chemical_compound Cell Wall Inner membrane General Materials Science chemistry.chemical_classification Reactive oxygen species Bacteria Superoxide 021001 nanoscience & nanotechnology Anti-Bacterial Agents 0104 chemical sciences Teichoic Acids chemistry Biophysics Lipoteichoic acid Cell envelope Reactive Oxygen Species 0210 nano-technology |
| Zdroj: | ACS Applied Materials & Interfaces. 12:5557-5568 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b20193 |
| Popis: | The work describes the interactions of nanosilver (NAg) with bacterial cell envelope components at a molecular level and how this associates with the reactive oxygen species (ROS)-mediated toxicity of the nanoparticle. Major structural changes were detected in cell envelope biomolecules as a result of damages in functional moieties, such as the saccharides, amides, and phosphodiesters. NAg exposure disintegrates the glycan backbone in the major cell wall component peptidoglycan, causes complete breakdown of lipoteichoic acid, and disrupts the phosphate-amine and fatty acid groups in phosphatidylethanolamine, a membrane phospholipid. Consistent with the oxidative attacks, we propose that the observed cell envelope damages are inflicted, at least in part, by the reactive oxygen radicals being generated by the nanoparticle during its leaching process, abiotically, without cells. The cell envelope targeting, especially those on the inner membrane phospholipid, is likely to then trigger the rapid generation of lethal levels of cellular superoxide (O2•-) and hydroxyl (OH•) radicals herein seen with a model bacterium. The present study provides a better understanding of the antibacterial mechanisms of NAg, whereby ROS generation could be both the cause and consequence of the toxicity, associated with the initial cell envelope targeting by the nanoparticle. |
| Databáze: | OpenAIRE |
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