Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8)/CLM-6 is an FcRγ-coupled receptor selectively expressed in mouse tissue plasmacytoid dendritic cells
| Autor: | Ayako Kaitani, Yoshinori Yamanishi, Keiko Maeda, Masamichi Isobe, Toshiyuki Takai, Nobuhiro Nakano, Ayako Takamori, Jiro Kitaura, Mariko Takahashi, Tomoaki Ando, Hiromichi Yamada, Koichiro Uchida, Akie Maehara, Masakazu Nagamine, Ko Okumura, Kumi Izawa, Toshio Kitamura, Shino Uchida, Toshihiro Matsukawa, Toshihiko Oki, Toshiaki Shimizu, Hideoki Ogawa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Spleen Stimulation Article Mice 03 medical and health sciences Bone Marrow Interferon Immune Tolerance medicine Animals Humans Receptors Immunologic Tyrosine lcsh:Science Receptor Mice Knockout Multidisciplinary biology Chemistry Receptors IgG lcsh:R TLR9 hemic and immune systems Dendritic Cells Flow Cytometry Cell biology Mice Inbred C57BL HEK293 Cells 030104 developmental biology medicine.anatomical_structure Organ Specificity Interferon Type I biology.protein lcsh:Q Lymph Nodes Bone marrow Antibody Biomarkers Signal Transduction medicine.drug |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Plasmacytoid dendritic cells (pDCs) produce large amounts of type-I interferon (IFN) in response to viral infection or self nucleic acids. Leukocyte mono-immunoglobulin-like receptor 8 (LMIR8), also called CMRF-35-like molecule-6 (CLM-6), is a putative activating receptor among mouse LMIR/CLM/CD300 members; however, the expression and function of LMIR8 remain unclear. Here, we characterize mouse LMIR8 as a pDC receptor. Analysis of Flag-tagged LMIR8-transduced bone marrow (BM)-derived mast cells demonstrated that LMIR8 can transmit an activating signal by interacting with immunoreceptor tyrosine-based activating motif (ITAM)-containing FcRγ. Flow cytometric analysis using a specific antibody for LMIR8 showed that LMIR8 expression was restricted to mouse pDCs residing in BM, spleen, or lymph node. FcRγ deficiency dampened surface expression of LMIR8 in mouse pDCs. Notably, LMIR8 was detected only in pDCs, irrespective of TLR9 stimulation, suggesting that LMIR8 is a suitable marker for pDCs in mouse tissues; LMIR8 is weakly expressed in Flt3 ligand-induced BM-derived pDCs (BMpDCs). Crosslinking of transduced LMIR8 in BMpDCs with anti-LMIR8 antibody did not induce IFN-α production, but rather suppressed TLR9-mediated production of IFN-α. Taken together, these observations indicate that LMIR8 is an FcRγ-coupled receptor selectively expressed in mouse tissue pDCs, which might suppress pDC activation through the recognition of its ligands. |
| Databáze: | OpenAIRE |
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