Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro
| Autor: | Lene C Poulsen, Espen S. Baekkevold, Berit Lyng Schiøtz, Siri Mjaaland, Tor Gjøen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Programmed cell death
Cell type Cell Survival Isavirus Salmo salar Caspase 3 Phosphatidylserines lcsh:Infectious and parasitic diseases Cell Line Immune system In vivo Interferon Virology medicine Animals lcsh:RC109-216 Caspase 7 biology Cell Death Research Cell Membrane biology.organism_classification Infectious salmon anemia virus Mitochondria Infectious Diseases Cell culture medicine.drug |
| Zdroj: | Virology Journal Virology Journal, Vol 6, Iss 1, p 91 (2009) |
| ISSN: | 1743-422X |
| Popis: | BackgroundInfectious salmon anemia virus (ISAV) is an aquatic orthomyxovirus and the causative agent of infectious salmon anemia (ISA), a disease of great importance in the Atlantic salmon farming industry.In vitro, ISAV infection causes cytophatic effect (CPE) in cell lines from Atlantic salmon, leading to rounding and finally detachment of the cells from the substratum. In this study, we investigated the mode of cell death duringin vitroISAV infection in different Atlantic salmon cell lines, using four ISAV strains causing different mortalityin vivo.ResultsThe results show that caspase 3/7 activity increased during the course of infection in ASK and SHK-1 cells, infected cells showed increased surface expression of phosphatidylserine and increased PI uptake, compared to mock infected cells; and morphological alterations of the mitochondria were observed. Expression analysis of immune relevant genes revealed no correlation between in vivo mortality and expression, but good correlation in expression of interferon genes.ConclusionResults from this study indicate that there is both strain and cell type dependent differences in the virus-host interaction during ISAV infection. This is important to bear in mind when extrapolatingin vitrofindings to thein vivosituation. |
| Databáze: | OpenAIRE |
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