Allogeneic transplantation of mobilized dental pulp stem cells with the mismatched dog leukocyte antigen type is safe and efficacious for total pulp regeneration

Autor: Koichiro Iohara, Misako Nakashima, Shinji Utsunomiya, Sakae Kohara
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Pathology
medicine.medical_specialty
Allogeneic transplantation
Pulpectomy
Medicine (miscellaneous)
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Pulp regeneration
Mobilized dental pulp stem cells
lcsh:Biochemistry
03 medical and health sciences
Dogs
stomatognathic system
Dental pulp stem cells
Granulocyte Colony-Stimulating Factor
Medicine
Autologous transplantation
Animals
Humans
Regeneration
Transplantation
Homologous
lcsh:QD415-436
Dual transplantation
Dental Pulp
Cell Proliferation
lcsh:R5-920
biology
business.industry
Dog leukocyte antigen
Research
Stem Cells
Allogeneic cell transplantation
Cell Differentiation
Cell Biology
Granulocyte-colony stimulating factor
Granulocyte colony-stimulating factor
Transplantation
stomatognathic diseases
030104 developmental biology
biology.protein
Molecular Medicine
Pulp (tooth)
Female
Stem cell
business
lcsh:Medicine (General)
Stem Cell Transplantation
Zdroj: Stem Cell Research & Therapy
Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-16 (2018)
ISSN: 1757-6512
Popis: Background We recently demonstrated that autologous transplantation of mobilized dental pulp stem cells (MDPSCs) was a safe and efficacious potential therapy for total pulp regeneration in a clinical study. The autologous MDPSCs, however, have some limitations to overcome, such as limited availability of discarded teeth from older patients. In the present study, we investigated whether MDPSCs can be used for allogeneic applications to expand their therapeutic use. Methods Analysis of dog leukocyte antigen (DLA) was performed using polymerase chain reaction from blood. Canine allogeneic MDPSCs with the matched and mismatched DLA were transplanted with granulocyte-colony stimulating factor in collagen into pulpectomized teeth respectively (n = 7, each). Results were evaluated by hematoxylin and eosin staining, Masson trichrome staining, PGP9.5 immunostaining, and BS-1 lectin immunostaining performed 12 weeks after transplantation. The MDPSCs of the same DLA used in the first transplantation were further transplanted into another pulpectomized tooth and evaluated 12 weeks after transplantation. Results There was no evidence of toxicity or adverse events of the allogeneic transplantation of the MDPSCs with the mismatched DLA. No adverse event of dual transplantation of the MDPSCs with the matched and mismatched DLA was observed. Regenerated pulp tissues including neovascularization and neuronal extension were quantitatively and qualitatively similar at 12 weeks in both matched and mismatched DLA transplants. Regenerated pulp tissue was similarly observed in the dual transplantation as in the single transplantation of MDPSCs both with the matched and mismatched DLA. Conclusions Dual allogeneic transplantation of MDPSCs with the mismatched DLA is a safe and efficacious method for total pulp regeneration. Electronic supplementary material The online version of this article (10.1186/s13287-018-0855-8) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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