Immaturity of IL-18 gene expression and protein production in cord blood (CB) versus peripheral blood (PB) mononuclear cells and differential effects in natural killer (NK) cell development and function*
| Autor: | Janet Ayello, Mitchell S. Cairo, Lynn L. Simpson, Carmella van de Ven, Laxmi V. Baxi, Allison F. O'Neill, Prakash Satwani |
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| Rok vydání: | 2005 |
| Předmět: |
Cytotoxicity
Immunologic Cell Survival Gene Expression Apoptosis Biology Peripheral blood mononuclear cell Natural killer cell Blood cell Interferon-gamma Gene expression medicine Humans Interferon gamma RNA Messenger Cells Cultured Reverse Transcriptase Polymerase Chain Reaction Interleukin-18 Hematology Fetal Blood Interleukin-12 Molecular biology Killer Cells Natural medicine.anatomical_structure Cord blood Immunology Leukocytes Mononuclear Interleukin 12 Interleukin 18 Cell Division Half-Life medicine.drug |
| Zdroj: | British Journal of Haematology. 130:284-292 |
| ISSN: | 1365-2141 0007-1048 |
| DOI: | 10.1111/j.1365-2141.2005.05592.x |
| Popis: | We have previously demonstrated dysregulation of IL-12 and IL-15 gene and protein expression between activated cord blood (CB) versus peripheral blood (PB) mononuclear cells (MNCs). In the present study, we compared IL-18 gene expression and protein production and IL-18 mRNA half-life in basal versus activated CB versus PB MNCs, the effects of IL-18 +/- IL-12 on MNCs IFN-gamma protein production and ex vivo expansion and activation of CB with IL-12 + IL-2 + anti-CD3 +/- IL-18. Basal and activated levels of IL-18 were significantly higher in PB versus CB MNCs (P < 0.05). IL-18 mRNA was coincidental with protein levels and significantly lower in CB (P < 0.05) and its half-life significantly shorter in CB versus PB MNCs (P < 0.05). IL-18 synergistically with IL-12 induced IFN-gamma production from PB greater than CB MNCs (P < 0.05). NK cells expansion (P < 0.001) and cytotoxicity (P < 0.01) was significantly increased with IL-12 + IL-2 + anti-CD3 and IL-18. In summary IL-18 gene expression and protein production are significantly decreased in activated CB versus PB MNCs, in part secondary to increased degradation of CB IL-18 mRNA. These results may have implications for the mechanism(s) in part responsible for the immaturity of CB T-cell immunity. |
| Databáze: | OpenAIRE |
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