Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops
| Autor: | Livia Stavolone, Steve Milligan, Thomas Hohn, Kay Lawton, Peter de Haan, A. Ragozzino, Sandra Pauli, Maria Kononova |
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| Přispěvatelé: | Stavolone, L., Kononova, M., Pauli, S., Ragozzino, Antonio, DE HAAN, P., Milligan, S., Lawton, K., Hohn, T. |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Transgene
Nicotiana tabacum Recombinant Fusion Proteins Green Fluorescent Proteins Arabidopsis Gene Dosage Gene Expression Enzyme-Linked Immunosorbent Assay Plant Science Biology Zea mays Lycopersicon Solanum lycopersicum Transcription (biology) Caulimovirus Botany Gene expression Tobacco Genetics Transgenes Promoter Regions Genetic Glucuronidase Histocytochemistry Cestrum fungi food and beverages Promoter Oryza General Medicine Meristem biology.organism_classification Plants Genetically Modified Luminescent Proteins Microscopy Fluorescence Caulimoviridae Agronomy and Crop Science Plasmids |
| Popis: | Appropriately regulated gene expression requires a suitable promoter. A number of promoters have been isolated and shown to be functional in plants, but only a few of them activate transcription of transgenes at high levels constitutively. We report here the cloning and characterization of a novel, constitutively expressed promoter isolated from Cestrum yellow leaf curling virus (CmYLCV), a double-stranded DNA plant pararetrovirus belonging to the Caulimoviridae family. The CmYLCV promoter is highly active in callus, meristems and vegetative and reproductive tissues in Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Zea mays and Oryza sativa. Furthermore, the level of expression is comparable to, or higher than, that from the CaMV 35S, the 'super-promoter' or the maize ubiquitin 1 promoters, three frequently used promoters in agricultural biotechnology. The heritable, strong and constitutive activity in both monocotyledonous and dicotyledonous plants, combined with the extremely narrow CmYLCV host range, makes the CmYLCV promoter an attractive tool for regulating transgene expression in a wide variety of plant species. |
| Databáze: | OpenAIRE |
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