Poisoning with methanol and ethylene glycol: 1H NMR spectroscopy as an effective clinical tool for diagnosis and quantification
| Autor: | Michel Imbenotte, Gaston Vermeersch, Anne Wahl, Gérard Forzy, Bernard Cartigny, Daniel Mathieu, Michel Lhermitte, Nathalie Azaroual |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Chromatography Gas Magnetic Resonance Spectroscopy Analytical chemistry Toxicology chemistry.chemical_compound Biotransformation medicine Humans Formate Aged Chromatography Ethanol Methanol Poisoning Metabolic acidosis Middle Aged medicine.disease chemistry Proton NMR Ethylene Glycols Female Acidosis Ethylene glycol Quantitative analysis (chemistry) |
| Zdroj: | Toxicology. 128:73-81 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/s0300-483x(98)00055-9 |
| Popis: | Analysis of serum and urine samples from three patients in a metabolic acidotic state was performed using biochemical methods and proton nuclear magnetic resonance (1H NMR). Methanol and ethylene glycol were identified by their singlet peaks in the 1H NMR spectra recorded directly from 0.5 ml of the collected samples. Quantification of these compounds was obtained simultaneously with that of their metabolites, formate and glycolate respectively and of lactate and ethanol, the latter being used as an antidote. The NMR results were found to be very similar to the biochemical findings. The results presented here suggest that 1H NMR can be clinically useful since it quickly provides information on the onset of metabolic acidosis and on the biotransformation of xenobiotics. |
| Databáze: | OpenAIRE |
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