Nuclear ribonucleoprotein complexes containing U1 and U2 RNA
| Autor: | Tae Suk Ro-Choi, Raj Nb, Harris Busch |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Cell Nucleus
Male Carcinoma Hepatocellular RNase P Chemistry Liver Neoplasms Ribonucleoprotein particle Oligonucleotides RNA Neoplasms Experimental Heterogeneous ribonucleoprotein particle Biochemistry Chromatin Rats Sepharose Nucleoproteins Ribonucleases Ribonucleoproteins Animals Centrifugation Electrophoresis Polyacrylamide Gel RNA Neoplasm Polyacrylamide gel electrophoresis Ribonucleoprotein |
| Zdroj: | Biochemistry. 14(20) |
| ISSN: | 0006-2960 |
| Popis: | Nuclear ribonucleoprotein (RNP) complexes that contain the U1 and U2 RNA of chromatin of Novikoff hepatoma cells were extracted with 0.01 M Tris-HCl (pH 8.0) after the nuclei were initially washed with 0.075 M NaCl and 0.025 M EDTA (pH 8.0). These RNP complexes were purified by chromatography on Sepharose 6B columns and centrifugation on sucrose density gradients. The identity of the U1 and U2 RNA in these particles was established by their electrophoretic mobility in polyacrylamide gels and their T1 RNase fingerprints which were identical with those of authentic U1 and U2 RNA (R. Reddy et al. (1974), J. Biol. Chem.249, 6486-6494; H. Shibata et al. (1974), Mol. Cell. Biochem. 4, 3-19). The nuclear riboncleoproteins had a buoyant density of 1.47 g/ml in CsCl gradients. Two-dimensional polyacrylamide gel electrophoresis of their proteins showed these RNP complexes contain 10 polypeptide spots, of which two are phosphorylated in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|