Penetration and intracellular uptake of poly(glycerol-adipate) nanoparticles into three-dimensional brain tumour cell culture models
| Autor: | Martin C. Garnett, David Walker, Weina Meng, Terence L. Parker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Polyesters Population Cell Cell Culture Techniques Nanotechnology 02 engineering and technology Models Biological General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Organ Culture Techniques In vivo medicine Animals Humans Rats Wistar education Original Research education.field_of_study Drug Carriers Chemistry Brain Neoplasms 021001 nanoscience & nanotechnology In vitro 030104 developmental biology medicine.anatomical_structure Cell culture Drug delivery Biophysics Nanoparticles 0210 nano-technology Drug carrier Intracellular |
| Popis: | Nanoparticle (NP) drug delivery systems may potentially enhance the efficacy of therapeutic agents. It is difficult to characterize many important properties of NPs in vivo and therefore attempts have been made to use realistic in vitro multicellular spheroids instead. In this paper, we have evaluated poly(glycerol-adipate) (PGA) NPs as a potential drug carrier for local brain cancer therapy. Various three-dimensional (3-D) cell culture models have been used to investigate the delivery properties of PGA NPs. Tumour cells in 3-D culture showed a much higher level of endocytic uptake of NPs than a mixed normal neonatal brain cell population. Differences in endocytic uptake of NPs in 2-D and 3-D models strongly suggest that it is very important to use in vitro 3-D cell culture models for evaluating this parameter. Tumour penetration of NPs is another important parameter which could be studied in 3-D cell models. The penetration of PGA NPs through 3-D cell culture varied between models, which will therefore require further study to develop useful and realistic in vitro models. Further use of 3-D cell culture models will be of benefit in the future development of new drug delivery systems, particularly for brain cancers which are more difficult to study in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|