Identification and validation of a novel autophagy gene expression signature for human bladder cancer patients
| Autor: | Sanaa Eissa, Yousif Kotb, Nahla M Awad, Marwa Matboli |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Urinary system Urinary Bladder Human bladder Biology Bioinformatics 03 medical and health sciences 0302 clinical medicine Gene expression Autophagy Biomarkers Tumor medicine Autophagy-Related Protein-1 Homolog Humans In patient RC254-282 Aged Urine cytology Bladder cancer medicine.diagnostic_test Intracellular Signaling Peptides and Proteins Neoplasms. Tumors. Oncology. Including cancer and carcinogens Computational Biology Membrane Proteins Cancer General Medicine Middle Aged medicine.disease DNA-Binding Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Urinary Bladder Neoplasms 030220 oncology & carcinogenesis Cancer research Female Transcriptome Microtubule-Associated Proteins Autophagy-Related Protein 12 Transcription Factors |
| Zdroj: | Tumor Biology, Vol 39 (2017) |
| ISSN: | 1423-0380 1010-4283 |
| Popis: | We sought to identify and validate a novel urinary autophagy transcript signature in patients with bladder cancer and evaluate its clinical utility. We performed an initial screening for seven autophagy transcript–based panel (autophagy-related protein 12 (ATG12); WD repeat domain, phosphoinositide interacting 2 (WIPI2); FYVE and coiled-coil domain-containing protein 1 (FYCO1); microtubule-associated protein light chain (MAPLC3); RB1-inducible coiled-coil 1 (RB1CC1); tachylectin-II-like beta-propeller domain 1 (TECPR1); and Unc-51-like kinase (ULK1)) that was identified based on bioinformatics analysis followed by SYBR Green–based polymerase chain reaction array validation in paired tissue and urine samples. Afterward, we evaluated the expression of differentially expressed autophagy transcripts in an independent validation set with reverse transcription quantitative real-time polymerase chain reaction in urine sediments of 140 patients with bladder cancer, 68 patients with benign urological lesions, and 74 healthy controls (age and sex matched). The expression levels of ATG12, FYCO1, TECPR1, and ULK1 in paired bladder tissue and urine samples were significantly lower in bladder cancer than in control group (p |
| Databáze: | OpenAIRE |
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