Effect of storage tube material and resveratrol during liquid storage of matured bovine oocytes on subsequent development
| Autor: | S. Matoba, Tamas Somfai, Rangsun Parnpai, Takashi Nagai, Tayita Suttirojpattana, Masaya Geshi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Fertilization in Vitro Resveratrol Antioxidants Andrology 03 medical and health sciences chemistry.chemical_compound Tissue culture Stilbenes medicine Animals Blastocyst Liquid storage General Veterinary Chemistry Embryogenesis Oocyte In Vitro Oocyte Maturation Techniques 030104 developmental biology medicine.anatomical_structure embryonic structures Oocytes Cattle Tissue Preservation |
| Zdroj: | SC30201804040025 NARO成果DBa 書誌情報のみ |
| ISSN: | 0236-6290 |
| DOI: | 10.1556/004.2017.053 |
| Popis: | This study determined the optimum storage vessel and the effects of resveratrol for the storage of in vitro matured (IVM) bovine oocytes. After IVM, the oocytes were kept in a Hepes-buffered medium at 25 °C for 20 h in different containers including Eppendorf tubes (ET) made of polypropylene (PP) and polystyrene (PS), and tissue culture tubes (TCT) made of PP, PS, and glass. Then oocytes were subjected to IVF and subsequent in vitro embryo development was compared among the groups and to that of a control group without storage. The percentage of blastocyst development in the control group was significantly higher than in the stored groups (P < 0.05). Among oocytes stored in TCT, the percentage of blastocyst development of oocytes stored in glass TCT was significantly higher than that of oocytes stored in PP and PS TCT (P < 0.05); however, it did not differ from that of oocytes stored in ET. The quality of blastocysts did not differ among the control and stored groups. Embryo development was not affected when 0.1, 1 or 10 μM resveratrol was added to the medium during oocyte storage. In conclusion, glass tubes were optimal for oocyte storage and resveratrol did not improve the development of stored oocytes. |
| Databáze: | OpenAIRE |
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