Residual γH2AX foci induced by low dose x-ray radiation in bone marrow mesenchymal stem cells do not cause accelerated senescence in the progeny of irradiated cells
| Autor: | Sergey V. Leonov, Alex Zhavoronkov, I. Kobzeva, Dmitry Klokov, Тatiana Karaseva, Anna Grekhova, V. Brunchukov, Taisia Blokhina, Victoria Nikitina, D. Usupzhanova, Yulia Suchkova, Natalia Vorobyeva, Тatiana A. Astrelina, Anastasia Tsvetkova, Andreyan N. Osipov, Aleksandr Samoylov, Evgeny Izumchenko, Ivan V. Ozerov, Margarita Pustovalova, Andrey Bushmanov |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Senescence Adult Male Aging Time Factors Bone Marrow Cells Radiation Ionizing radiation Histones 03 medical and health sciences 0302 clinical medicine cellular senescence delayed radiation effects Humans Irradiation Cells Cultured Cell Proliferation cellular proliferation Chemistry Cell growth X-Rays Mesenchymal stem cell X-ray low-dose radiation effects Dose-Response Relationship Radiation Mesenchymal Stem Cells Cell Biology beta-Galactosidase Molecular biology γH2AX foci 030104 developmental biology Ki-67 Antigen 030220 oncology & carcinogenesis Signal transduction Research Paper Signal Transduction |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Mechanisms underlying the effects of low-dose ionizing radiation (IR) exposure (10-100 mGy) remain unknown. Here we present a comparative study of early (less than 24h) and delayed (up to 11 post-irradiation passages) radiation effects caused by low (80 mGy) vs intermediate (1000 mGy) dose X-ray exposure in cultured human bone marrow mesenchymal stem cells (MSCs). We show that γН2АХ foci induced by an intermediate dose returned back to the control value by 24 h post-irradiation. In contrast, low-dose irradiation resulted in residual γН2АХ foci still present at 24 h. Notably, these low dose induced residual γН2АХ foci were not co-localized with рАТМ foci and were observed predominantly in the proliferating Кi67 positive (Кi67+) cells. The number of γН2АХ foci and the fraction of nonproliferating (Кi67-) and senescent (SA-β-gal+) cells measured at passage 11 were increased in cultures exposed to an intermediate dose compared to unirradiated controls. These delayed effects were not seen in the progeny of cells that were irradiated with low-dose X-rays, although such exposure resulted in residual γН2АХ foci in directly irradiated cells. Taken together, our results support the hypothesis that the low-dose IR induced residual γH2AХ foci do not play a role in delayed irradiation consequences, associated with cellular senescence in cultured MSCs. |
| Databáze: | OpenAIRE |
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