Micro-needle implantable electrochemical oxygen sensor: ex-vivo and in-vivo studies
| Autor: | Elisenda Eixarch, Sandrine Miserere, Josep Samitier, Mònica Mir, Sergio Berdún Marin, Lourdes Rivas, Laura Pla, Johanna Parra, Samuel Dulay, Miriam Illa, Eduard Gratacós |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Biomedical Engineering Biophysics chemistry.chemical_element 02 engineering and technology Biosensing Techniques 01 natural sciences Oxygen Electricity Electrònica mèdica In vivo medicine Electrochemistry Animals Humans Electrodes Platinum Hyperoxia Elèctrodes 010401 analytical chemistry General Medicine Partial pressure Electrochemical Techniques Oxigen 021001 nanoscience & nanotechnology Gas analyzer 0104 chemical sciences Electrochemical gas sensor Electrodes Implanted Medical electronics Electroquímica Fluorocarbon Polymers Biosensors chemistry Needles Hypoxia-Ischemia Brain Rabbits medicine.symptom 0210 nano-technology Oxygen sensor Microelectrodes Ex vivo Biotechnology Biomedical engineering |
| Popis: | Oxygen is vital for energy metabolism in mammals and the variability of the concentration is considered a clinical alert for a wide range of metabolic malfunctions in medicine. In this article, we describe the development and application of a micro-needle implantable platinum-based electrochemical sensor for measuring partial pressure of oxygen in intramuscular tissue (in-vivo) and vascular blood (ex-vivo). The Pt-Nafion® sensor was characterized morphological and electrochemically showing a higher sensitivity of −2.496 nA/mmHg (−1.495 nA/μM) when comparing with its bare counterpart. Our sensor was able to discriminate states with different oxygen partial pressures (pO2) for ex-vivo (blood) following the same trend of the commercial gas analyzer used as standard. For in-vivo (intramuscular) experiments, since there is not a gold standard for measuring pO2 in tissue, it was not possible to correlate the obtained currents with the pO2 in tissue. However, our sensor was able to detect clear statistical differences of O2 between hyperoxia and hypoxia states in tissue. |
| Databáze: | OpenAIRE |
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