Improving the Diagnostic Accuracy of the PD-L1 Test with Image Analysis and Multiplex Hybridization

Autor:	Jacqueline James, Matthew P. Humphries, Stephen McQuaid, Stephanie G Craig, Manuel Salto-Tellez, Fatima Abdullahi Sidi, Victoria Bingham
Rok vydání:	2020
Předmět:	0301 basic medicine Oncology PD-L1 Cancer Research medicine.medical_specialty Concordance Diagnostic accuracy Immunofluorescence lcsh:RC254-282 Article 03 medical and health sciences Cytokeratin 0302 clinical medicine SDG 3 - Good Health and Well-being image analysis Internal medicine medicine Multiplex multiplexing medicine.diagnostic_test biology business.industry lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens clinical workflow 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Diagnostic assessment business
Zdroj:	Cancers Humphries, M P, Bingham, V, Abdullahi Sidi, F, Craig, S G, McQuaid, S, James, J & Salto-Tellez, M 2020, ' Improving the Diagnostic Accuracy of the PD-L1 Test with Image Analysis and Multiplex Hybridization ', Cancers, vol. 12, no. 5, 1114 . https://doi.org/10.3390/cancers12051114 Cancers, Vol 12, Iss 1114, p 1114 (2020) Volume 12 Issue 5
ISSN:	2072-6694
Popis:	Targeting of the programmed cell death protein (PD-1)/programmed death-ligand 1 (PD-L1) axis with checkpoint inhibitors has changed clinical practice in non-small cell lung cancer (NSCLC). However, clinical assessment remains complex and ambiguous. We aim to assess whether digital image analysis (DIA) and multiplex immunofluorescence can improve the accuracy of PD-L1 diagnostic testing. A clinical cohort of routine NSCLC patients reflex tested for PD-L1 (SP263) immunohistochemistry (IHC), was assessed using DIA. Samples of varying assessment difficulty were assessed by multiplex immunofluorescence. Sensitivity, specificity, and concordance was evaluated between manual diagnostic evaluation and DIA for chromogenic and multiplex IHC. PD-L1 expression by DIA showed significant concordance (R² = 0.8248) to manual assessment. Sensitivity and specificity was 86.8% and 91.4%, respectively. Evaluation of DIA scores revealed 96.8% concordance to manual assessment. Multiplexing enabled PD-L1+/CD68+ macrophages to be readily identified within PD-L1+/cytokeratin+ or PD-L1-/cytokeratin+ tumor nests. Assessment of multiplex vs. chromogenic IHC had a sensitivity and specificity of 97.8% and 91.8%, respectively. Deployment of DIA for PD-L1 diagnostic assessment is an accurate process of case triage. Multiplex immunofluorescence provided higher confidence in PD-L1 assessment and could be offered for challenging cases by centers with appropriate expertise and specialist equipment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98e8e9bbbe488214ed9984ad52c8e212 https://pubmed.ncbi.nlm.nih.gov/32365629 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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