Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability
| Autor: | Farah Hanan Fatihah Jaafar, Fazly Ann Zainalabidin, Siti Fatimah Ibrahim, Abas Mazni Othman, Mohd Iswadi Ismail, Nur Azianie Abd Ghani, Khairul Osman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Slow freezing
Cryopreservation endocrine system Multidisciplinary biology urogenital system canister viability lcsh:Biotechnology Semen Anatomy biology.organism_classification Bull semen Sperm Electroejaculation sperm Bali cattle Andrology motility lcsh:TP248.13-248.65 Sperm motility reproductive and urinary physiology |
| Zdroj: | Brazilian Archives of Biology and Technology, Vol 57, Iss 2, Pp 194-199 (2014) Brazilian Archives of Biology and Technology, Volume: 57, Issue: 2, Pages: 194-199, Published: APR 2014 Brazilian Archives of Biology and Technology v.57 n.2 2014 Brazilian Archives of Biology and Technology Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
| ISSN: | 1678-4324 |
| Popis: | The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm2) and single tubing-type canister (4π x 90 mm2). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p |
| Databáze: | OpenAIRE |
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