Interferon γ Gene Expression in Sensory Neurons: Evidence for Autocrine Gene Regulation
| Autor: | Elke Wilharm, Hartmut Wekerle, Lüder Behrens, Harald Neumann, Hannes Schmidt |
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| Rok vydání: | 1997 |
| Předmět: |
Transcription
Genetic G protein Immunology Gene Expression Biology Polymerase Chain Reaction Article Interferon-gamma Dorsal root ganglion Ganglia Spinal Gene expression medicine Animals Immunology and Allergy Microscopy Phase-Contrast Interferon gamma Neurons Afferent Autocrine signalling Receptor Cells Cultured Receptors Interferon Regulation of gene expression Gene Expression Regulation Developmental Molecular biology Rats DNA-Binding Proteins STAT1 Transcription Factor medicine.anatomical_structure Trans-Activators Signal transduction Signal Transduction medicine.drug |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.186.12.2023 |
| Popis: | We explored expression and possible function of interferon-γ (IFN-γ) in cultured fetal (E15) rat dorsal root ganglion neurons combining whole cell patch-clamp electrophysiology with single cell reverse transcriptase polymerase chain reaction and confocal laser immunocytochemistry. Morphologically, we located IFN-γ protein in the cytoplasm of the neurons in culture as well as in situ during peri- and postnatal development. Transcripts for classic IFN-γ and for its receptor were determined in probes of cytoplasm sampled from individual cultured neurons, which had been identified by patch clamp electrophysiology. In addition, the cultured neurons expressed both chains of the IFN-γ receptor. Locally produced IFN-γ acts back on its cellular source. Phosphorylation and nuclear translocation of the IFN-inducible transcriptional factor STAT1 as well as IFN-γ–dependent expression of major histocompatibility complex class I molecules on the neuronal membrane were noted in untreated cultures. However, both processes were substantially blocked in the presence of antibodies neutralizing IFN-γ. Our findings indicate a role of IFN-γ in autocrine regulation of sensory neurons. |
| Databáze: | OpenAIRE |
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