Aquaporin-mediated reduction in maize root hydraulic conductivity impacts cell turgor and leaf elongation even without changing transpiration
| Autor: | Christophe Maurel, François Tardieu, Thierry Simonneau, Christina Ehlert |
|---|---|
| Přispěvatelé: | Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
0106 biological sciences
Stomatal conductance Lpr MESH: Hydrogen-Ion Concentration Physiology MESH: Zea mays MESH: Plant Transpiration Turgor pressure water MESH: Plant Roots Plant Science Root system Biology maize 01 natural sciences MESH: Acids 03 medical and health sciences MESH: Aquaporins MESH: Xylem MESH: Anaerobiosis Genetics MESH: Water [SDV.BV]Life Sciences [q-bio]/Vegetal Biology skin and connective tissue diseases abiotic stimuli 030304 developmental biology Transpiration 2. Zero hunger MESH: Osmosis 0303 health sciences leaf MESH: Time Factors Xylem Plant physiology food and beverages hydroponics Hydroponics aquaporin MESH: Plant Leaves Horticulture Agronomy Root MESH: Hydrogen Peroxide Elongation MESH: Pressure MESH: Plant Stomata 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2009, 150 (2), pp.1093-104. ⟨10.1104/pp.108.131458⟩ |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.108.131458⟩ |
| Popis: | This work was supported by the Institut National de la Recherche Agronomique (doctoral fellowship to C.E. and grant no. AIP300), the French Ministry of Research (grant no. ACI2003 [Biologie du Développement et Physiologie Intégrative] to C.M. and T.S.), and Agence Nationale de la Recherche Génoplante (award to F.T.).; International audience; Root hydraulic conductivity in plants (Lp(r)) exhibits large variations in response to abiotic stimuli. In this study, we investigated the impact of dynamic, aquaporin-mediated changes of Lp(r) on leaf growth, water potential, and water flux throughout the plant. For this, we manipulated Lp(r) by subjecting roots to four independent treatments, with aquaporin inhibitors applied either to transpiring maize (Zea mays) plants grown in hydroponics or to detopped root systems for estimation of Lp(r). The treatments were acid load at pH 6.0 and 5.0 and hydrogen peroxide and anoxia applied for 1 to 2 h and subsequently reversed. First, we established that acid load affected cell hydraulic conductivity in maize root cortex. Lp(r) was reduced by all treatments by 31% to 63%, with half-times of about 15 min, and partly recovered when treatments were reversed. Cell turgor measured in the elongating zone of leaves decreased synchronously with Lp(r), and leaf elongation rate closely followed these changes across all treatments in a dose-dependent manner. Leaf and xylem water potentials also followed changes in Lp(r). Stomatal conductance and rates of transpiration and water uptake were not affected by Lp(r) reduction under low evaporative demand. Increased evaporative demand, when combined with acid load at pH 6.0, induced stomatal closure and amplified all other responses without altering their synchrony. Root pressurization reversed the impact of acid load or anoxia on leaf elongation rate and water potential, further indicating that changes in turgor mediated the response of leaf growth to reductions in Lp(r). |
| Databáze: | OpenAIRE |
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