Workflow of Lotmaria passim isolation: Experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain
| Autor: | María Buendía-Abad, Raquel Martín-Hernández, Luis Miguel De Pablos, Laura Barrios, Mariano Higes, Alberto Fernández Fernández, Antonio Osuna, Aránzazu Meana |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lotmaria passim Honeybee 030231 tropical medicine Virulence Isolation Microbiology 03 medical and health sciences 0302 clinical medicine Parasite hosting Experimental infection Axenic Trypanosomatid biology Mortality rate Strain (biology) 030108 mycology & parasitology Isolation (microbiology) biology.organism_classification Pathogenicity Low-passage Infectious Diseases QL1-991 Animal Science and Zoology Parasitology Zoology |
| Zdroj: | International Journal for Parasitology: Parasites and Wildlife, Vol 14, Iss, Pp 68-74 (2021) |
| ISSN: | 2213-2244 |
| Popis: | The impact of trypanosomatid parasites on honeybee health may represent a major threat to bee colonies worldwide. However, few axenic isolates have been generated to date and with no details on cell culture passages, a parameter that could influence parasite virulence. To address this question, a trypanosomatid isolation protocol was developed and a new strain was obtained, named L. passim C1. Using experimental infection of worker honeybees, we compared the virulence and mortality rates of the ATCC PRA-403 reference strain and C1 strain, the latter showing higher virulence from 10 days post-infection onward. This study highlights the impact of cell culture passages on the pathogenicity of L. passim in honeybees, providing new evidence of its negative effects on honeybee health. |
| Databáze: | OpenAIRE |
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