A comparison of the properties of bacteriocins formed by Lactococcus lactis subsp. lactis strains of diverse origin
| Autor: | L G Stoianova, N. S. Egorov, G. B. Fedorova, Alexander I. Netrusov, G. S. Katrukha |
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| Rok vydání: | 2007 |
| Předmět: |
Strain (chemistry)
Mutant Lactococcus lactis food and beverages biochemical phenomena metabolism and nutrition Biology Protoplast Antimicrobial biology.organism_classification Applied Microbiology and Biotechnology Biochemistry law.invention Microbiology chemistry.chemical_compound Bacteriocin chemistry law Recombinant DNA bacteria Nisin |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | Bacteriocins formed by four strains of Lactococcus lactis subsp. lactis have been studied and compared: 729 (a natural strain isolated from milk), 1605 (a mutant of strain 729), F-116 (a recombinant obtained by fusing of protoplasts of the two related strain 729 and 1605), and a nisin-forming strain obtained by adaptive selection at Moscow State University. Antimicrobial activity studies revealed differences between the strains in the effects on individual groups of microorganisms; the activities of the strains were also distinct from that of Nisaplin (a commercial preparation of the bacteriocin nisin). Methods for isolation and purification of bacteriocins have been developed, making it possible to obtain individual components of antibiotic complexes as chromatographically pure preparations. Bacteriocins formed by the strains of Lactococcus lactis subsp. lactis have been identified and differences in their biological and physicochemical properties, established. A novel potent broad-spectrum antibiotic substance distinct from nisin has been isolated from the recombinant strain F-116. |
| Databáze: | OpenAIRE |
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