Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma
| Autor: | Mariann Rand-Weaver, Peter Thomas, Dionisis Mparmpakas, Elena Zachariades, Emmanouil Karteris |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
Time Factors Interleukin-1beta Biology Promegestone Pregnancy Cell Line Tumor Progesterone receptor Nitriles medicine Butadienes Tumor Microenvironment Humans Choriocarcinoma Phosphorylation Protein Kinase Inhibitors Progesterone Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Cell Death Membrane Proteins medicine.disease Interleukin 1β Oncology embryonic structures Immunology Uterine Neoplasms Cancer research Female Receptors Progesterone Signal Transduction |
| Zdroj: | International journal of oncology. 40(5) |
| ISSN: | 1791-2423 |
| Popis: | Copyright @ 2012 Spandidos Publications Ltd. This article can be accessed from the links below. This article has been made available through the Brunel Open Access Publishing Fund. Choriocarcinoma is a highly malignant epithelial tumour that is most often associated with hydatidiform mole and presents the most common emergency medical problem in the management of trophoblast disease. We hypothesise that the hormones/cytokines present within the tumour microenvironment play key roles in the development of choriocarcinoma. In this study we assessed the effects of interleukin-1β (IL-1β) on cell death in the presence or absence of the sex hormone progesterone using two choriocarcinoma cell lines (BeWo and JEG-3) as in vitro experimental models. Although IL-1β induced cell death in both cell lines, the effect was more pronounced in JEG-3 cells, where cell death reached 40% compared to 15% in BeWo cells. Cell death of JEG-3 cells in response to IL-1β was significantly decreased by co-treatment with 100 nM and 1000 nM progesterone and completely abolished at a progesterone concentration of 1000 nM. Progesterone was also able to induce phosphorylation of ERK1/2 in these cells. Pretreatment of JEG-3 cells with a specific MAPK inhibitor (UO126) inhibited progesterone's inhibitory effect on cell death. Collectively, these data provide evidence of cross-talk between progesterone and IL-1β in this aggressive and poorly understood tumour that involves activation of a MAPK pathway and involvement of numerous progesterone receptors. This research was funded by a National Institutes of Health Grant ESO12961. This article is made available through the Brunel Open Access Publishing Fund. |
| Databáze: | OpenAIRE |
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