Investigation of the Antibacterial Activity and
| Autor: | Monir Hossain, Masato Takikawa, Shinji Takeoka, Tanushree Saha, Ashraful Hasan, Sharif Hossain, Razib Datta Shubhra, Zinia Islam, Satya Ranjan Sarker, Shakil Ahmed Polash |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Histology lcsh:Biotechnology Biomedical Engineering Bioengineering 02 engineering and technology medicine.disease_cause Enterococcus faecalis Silver nanoparticle Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration biocompatibility CellToxTM green assay lcsh:TP248.13-248.65 medicine Original Research antimicrobial activity Chromatography biology Bioengineering and Biotechnology biomarkers lipid peroxidation pathogenic bacteria hemocompatibility 021001 nanoscience & nanotechnology biology.organism_classification 030104 developmental biology chemistry Staphylococcus aureus Trypan blue Liver function 0210 nano-technology Antibacterial activity biogenic silver nanoparticles Biotechnology |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 7 (2019) Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| Popis: | Biogenic nanoparticles are the smartest weapons to deal with the multidrug-resistant “superbugs” because of their broad-spectrum antibacterial propensity as well as excellent biocompatibility. The aqueous biogenic silver nanoparticles (Aq-bAgNPs) and ethanolic biogenic silver nanoparticles (Et-bAgNPs) were synthesized using aqueous and ethanolic extracts of Andrographis paniculata stem, respectively, as reducing agents. Electron microscopic images confirmed the synthesis of almost spherical shaped biogenic silver nanoparticles (bAgNPs). The zeta potentials of the nanoparticles were negative and were −22 and −26 mV for Aq-bAgNPs and Et-bAgNPs, respectively. The antibacterial activity of bAgNPs was investigated against seven pathogenic (i.e., enteropathogenic Escherichia coli, Salmonella typhi, Staphylococcus aureus, Vibrio cholerae, Enterococcus faecalis, Hafnia alvei, Acinetobacter baumannii) and three nonpathogenic (i.e., E. coli DH5α, E. coli K12, and Bacillus subtilis) bacteria at different time points (i.e., 12, 16, 20, and 24 h) in a dose-dependent manner (i.e., 20, 40, and 60 μg) through broth dilution assay, disk diffusion assay, CellToxTM Green uptake assay, and trypan blue dye exclusion assay. The lowest minimum inhibitory concentration value for both the bAgNPs was 0.125 μg. Et-bAgNPs showed the highest antibacterial activity against S. aureus at 60 μg after 16 h and the diameter of inhibited zone was 28 mm. Lipid peroxidation assay using all the bacterial strains revealed the formation of malondialdehyde–thiobarbituric acid adduct due to the oxidation of cell membrane fatty acids by bAgNPs. The bAgNPs showed excellent hemocompatibility against human as well as rat red blood cells. Furthermore, there was no significant toxicity observed when the levels of rat serum ALT, AST, γ-GT (i.e., liver function biomarkers), and creatinine (i.e., kidney function biomarker) were determined. |
| Databáze: | OpenAIRE |
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