Pathogenesis of Murine Lymphotropic Gammaherpesvirus Isolate 78
| Autor: | Mistríková J, M. Mrmusová-Šupolíková, M. Pappova |
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| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Acta Veterinaria Brno, Vol 72, Iss 3, Pp 371-376 (2003) |
| ISSN: | 1801-7576 0001-7213 |
| DOI: | 10.2754/avb200372030371 |
| Popis: | Mrmusova-·upolikova M., M. Pappova, J. Mistrikova: Pathogenesis of Murine Lymphotropic Gammaherpesvirus Isolate 78. Acta Vet. Brno 2003, 72: 371-376 BALB/c mice were infected intranasally with the mouse lymphotropic gammaherpesvirus isolate 78 (MHV-78). Infectious virus was detected in various organs of infected mice at different time intervals during a period of 180 days post infection (p.i.) by cocultivation with permissive VERO cells and indirect immunofluorescence using monoclonal antibodies prepared against MHV. We observed the highest infectious titer of MHV-78 during the acute infection on day 15 in lungs, and on days 7 and 15 in blood. At later intervals of infection the virus was detected in spleen, peritoneal macrophages, thymus, bone marrow and mammary glands. Furthermore, the analysis of differential white blood cell count of healthy/control and virus-infected BALB/c mice was followed. The obtained results showed that the infection with MHV-78 was accompanied with the changes in the number of leukocytes and quality of differential white blood cell count in correlation with the duration of infection. In comparison with control group, mice infected with MHV-78 had increased numbers of leukocytes with the higher numbers on days 15 and 122 p.i. and increased numbers of lymphocytes associated with the appearance of atypical leukocytes. In acute infection (up to day 30 p.i.) the frequency of atypical leukocytes increased, reaching a maximum at day 15 p.i. A similar increase in the frequency of these cells was observed in the course of persistent infection (after day 30 p.i.), reaching a maximum at day 122 p.i. Approximately 7% of BALB/c mice infected with MHV-78 developed tumors. Cells derived from tumor were positive for viral antigen.These results showed that MHV-78 is lymphotropic, oncogenic and very similar with MHV-68, MHV-72, MHV-76, MHV-·umava in some virological and pathogenetic features. Mouse herpesvirus isolate 78, pathogenesis, lymphatic system, atypical leukocytes |
| Databáze: | OpenAIRE |
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