Dietary K + acts as a genuine diuretic
| Autor: | Samuel L. Svendsen, Simon Kornvig, Peder Berg, Iben S. Jensen, Isabela B. B. A. de Araujo, Casper K. Larsen, Jens Leipziger, Mads V. Sørensen |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Svendsen, S L S C, Kornvig, S, Berg, P, Jensen, I S, Bastos Binotti Abreu De Araujo, I, Larsen, C K, Leipziger, J G & Sørensen, M V 2022, ' Dietary K + acts as a genuine diuretic ', Acta Physiologica (Online) . https://doi.org/10.1111/apha.13762 Svendsen, S L, Kornvig, S, Berg, P, Jensen, I S, de Araujo, I B B A, Larsen, C K, Leipziger, J & Sorensen, M V 2022, ' Dietary K + acts as a genuine diuretic ', Acta Physiologica, vol. 234, no. 2, e13762 . https://doi.org/10.1111/apha.13762 |
| DOI: | 10.1111/apha.13762 |
| Popis: | K+ balance in mammals relies on regulated renal K+ excretion matching unregulated fluctuating K+ intake. Upon a K+ rich meal, rapid and powerful K+ excretion is needed. Renal K+ secretion is stimulated by increased tubular flow. We speculated that high K+ intake acutely increases urinary flow to stimulate K+ excretion.Methods: Mice were K+ challenged through diets or gavage. Post K+ loading urinary output, osmolarity, [K+ ]u , [Na+ ]u , plasma osmolarity, [copeptin]p , [K+ ]p , and [Na+ ]p were measured. To locate the mechanism of K+ -induced diuresis in the glomerular/tubular system we measured creatinine excretion and assessed functional transport in isolated perfused TALs and CDs during an acute [K+ ]bl switch from 3.6 to 6.5 mM. Molecular adaptations of transport proteins involved in water reabsorption were investigated by immunoblotting.Results: 1) Mice switched from a 1% to 2% K+ diet increased diuresis within 12h and reciprocally reduced diuresis when switched from 1% to 0.01% K+ diet. 2) A single K+ gavage load, corresponding to 25-50% of daily K+ intake, induced 100% increase in diuresis within 30 minutes. This occurred despite augmented plasma osmolarity and AVP synthesis. 3) K+ gavage did not change GFR. 4) In isolated perfused TALs, shifting [K+ ]bl from 3.6 to 6.5 mM did not affect AVP-induced NaCl transport. 5) In sharp contrast, in isolated perfused CDs, shifting [K+ ]bl from 3.6 to 6.5 mM markedly reduced CD AVP-sensitivity, i.e. inhibited water absorption.Conclusion: Dietary K+ loading induces a rapidly on-setting diuresis. The mechanism of K+ -induced diuresis involves desensitization of the CD to AVP. |
| Databáze: | OpenAIRE |
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