Red blood cells: reservoirs of cis- and trans-epoxyeicosatrienoic acids
| Autor: | Houli Jiang, John C. McGiff, John Quilley, L. Manmohan Reddy, John R. Falck, Patrick Y.-K. Wong |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Erythrocytes Physiology Tandem mass spectrometry Epoxyeicosatrienoic acid Biochemistry High-performance liquid chromatography Rats Sprague-Dawley chemistry.chemical_compound 8 11 14-Eicosatrienoic Acid hemic and lymphatic diseases Animals Chromatography High Pressure Liquid Phospholipids Pharmacology Chromatography Stereoisomerism hemic and immune systems Cell Biology BSTFA Rats chemistry cardiovascular system lipids (amino acids peptides and proteins) Lipid Peroxidation Cis–trans isomerism circulatory and respiratory physiology |
| Zdroj: | Prostaglandins & Other Lipid Mediators. 75:65-78 |
| ISSN: | 1098-8823 |
| DOI: | 10.1016/j.prostaglandins.2004.10.003 |
| Popis: | Epoxyeicosatrienoic acids (EETs) are candidate endothelium-derived hyperpolarizing factors that demonstrate a wide range of biological effects. The presence of both cis - and trans -EETs in rat plasma was identified with HPLC-electrospray ionization tandem mass spectrometry in this study. The total EETs in plasma are 38.2 ng/ml with cis -EETs representing 21.4 ± 0.4 ng/ml and trans -EETs 16.8 ± 0.4 ng/ml. EETs in RBCs were estimated to be 20.2 ng/10 9 RBCs, which corresponds to 200 ng in RBCs contained in 1 ml blood. RBC incubation with 10 mM tert -butyl hydroperoxide resulted in 4.4-fold increase of total cis -EETs (from 9.2 to 40.2 ng/10 9 RBCs) and 5.5-fold increase of total trans -EETs (from 11.0 to 60.8 ng/10 9 RBCs). EETs were released (2 ng/ml) from RBCs after incubation at 37 °C for 10 min even after being washed 3 times, indicating that RBCs are reservoirs of plasma EETs. The identification of cis - and trans -EETs in RBCs and in plasma as well as their release from RBCs suggest a vasoregulatory role of RBCs in view of their potent vasoactivity. |
| Databáze: | OpenAIRE |
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