The effects of static pressure on chondrogenic and osteogenic differentiation in condylar chondrocytes from temporomandibular joint

Autor: Hui Li, Linjian Huang, Qianyang Xie, Xieyi Cai, Min Zhang, Chi Yang
Rok vydání: 2014
Předmět:
musculoskeletal diseases
medicine.medical_specialty
Indian hedgehog
Cell Survival
Blotting
Western
Core Binding Factor Alpha 1 Subunit
SOX9
Real-Time Polymerase Chain Reaction
Immunoenzyme Techniques
Chondrocytes
stomatognathic system
Western blot
Osteogenesis
Internal medicine
medicine
Pressure
Animals
Hedgehog Proteins
Viability assay
General Dentistry
Collagen Type II
medicine.diagnostic_test
biology
Temporomandibular Joint
Chemistry
Mandibular Condyle
Parathyroid Hormone-Related Protein
Cell Differentiation
SOX9 Transcription Factor
Cell Biology
General Medicine
Anatomy
musculoskeletal system
Chondrogenesis
biology.organism_classification
Alkaline Phosphatase
Temporomandibular joint
RUNX2
medicine.anatomical_structure
Endocrinology
Otorhinolaryngology
Microscopy
Electron
Scanning
Alkaline phosphatase
Rabbits
Zdroj: Archives of oral biology. 60(4)
ISSN: 1879-1506
Popis: Objective The goal of the study was to investigate the production of collagen, type II, alpha 1 (COL2A1), SOX9, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), Indian hedgehog (Ihh), and periarticular cell-derived parathyroid hormone-related protein (PTHrP) in mandibular condylar chondrocytes under static pressure stimuli. Methods Mandibular condylar chondrocytes separated from rabbit temporomandibular joint (TMJ) were treated with a static pressure of 100 kPa for 0, 1, 2, 3, and 4 h by an in-house-designed pressure chamber. A CCK-8 kit was used to analyze the cell viability. The production of COL2A1, SOX9, ALP, Runx2, Ihh, and PTHrP was detected by Western blot or real-time polymerase chain reaction (PCR). Changes in cell morphology were observed by scanning electron microscopy. Results Compared with the control group (0 h), the cytoplasmic processes of treated chondrocytes obviously increased and elongated, and the cell viability of pressurized chondrocytes were 91.13% (1 h), 103.41% (2 h), 103.47% (3 h), and 104.94% (4 h), respectively. The exposure of condylar chondrocytes to a static pressure of 100 kPa for 3–4 h resulted in a significant increase in COL2A1, SOX9, ALP, and Runx2. After a static pressure loading of 100 kPa, the activation of Ihh and PTHrP was also observed. Conclusions Mandibular condylar chondrocytes adapt to alterations of the microenvironment. Ihh and PTHrP are sensitive to static pressure. Our findings suggest that static pressure accelerated the chondrogenic and osteogenic differentiation of condylar chondrocytes, which may influence the pathological progress of temporomandibular diseases.
Databáze: OpenAIRE
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