In Vitro Antimicrobial Susceptibility of Pathogenic Leptospira Biofilm
| Autor: | I. P. Sunish, Kirubakaran Vinod Kumar, Kumaresan Vedhagiri, Ratchagadasse Vimal Raj, Chandan Lall, Paluru Vijayachari |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.drug_class Tetracycline 030106 microbiology Immunology Antibiotics Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences Antibiotic resistance Leptospira Ampicillin Drug Resistance Multiple Bacterial medicine Pharmacology Doxycycline Biofilm Penicillin G biochemical phenomena metabolism and nutrition biology.organism_classification Plankton Anti-Bacterial Agents Penicillin 030104 developmental biology Biofilms medicine.drug |
| Zdroj: | Microbial drug resistance (Larchmont, N.Y.). 22(7) |
| ISSN: | 1931-8448 |
| Popis: | Pathogenic Leptospira spp. are the causative agent of leptospirosis. Biofilm formation in leptospires is a new area of study, and its role in pathogenesis is not fully explored. As in other biofilm-forming bacteria, Leptospira biofilm may play a significant role in antibiotic resistance. In this study, the antimicrobial susceptibility of Leptospira biofilm was investigated by 96-well plate assay using Alamar Blue. Leptospira biofilm showed five to sixfold increase in resistance in all the strains used. The range of minimal bactericidal concentrations for penicillin G, ampicillin, tetracycline, and doxycycline was 1,600 U/ml, 800-1,600 μg/ml, 800-1,600 μg/ml, and 800-1,600 μg/ml, respectively. In agar substrate, the biofilm showed six- to sevenfold increase in resistance to antibiotics compared to planktonic cell. The present study emphasizes the importance of biofilm formation and its antibiotic susceptibility patterns. This could pave the way for devising appropriate strategy to prevent the occurrence of potential chronic leptospirosis in endemic areas and also during an outbreak situation. |
| Databáze: | OpenAIRE |
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