Embryogenesis and plant regeneration from isolated microspores of Brassica rapa L. ssp. Oleifera

Autor:	Laurie A. Burnett, Stephen Yarrow, Bin Huang
Rok vydání:	1992
Předmět:	education.field_of_study food.ingredient Regeneration (biology) Population Embryogenesis food and beverages Embryo Plant Science General Medicine Biology Tissue culture food Microspore Botany Brassica rapa education Canola Agronomy and Crop Science
Zdroj:	Plant Cell Reports. 11
ISSN:	1432-203X 0721-7714
Popis:	Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspore embryogenesis, buds (2-3mm in length, containing mid-late uninucieate microspores) were collected from older plants (2 months old) and microspores isolated and washed in iron-free B5 medium. NLN medium with its iron content reduced to half was beneficial for initial microspore culture. An elevated temperature(33-35°C) during the first day of culture, followed by maintenance at 25°C resulted in dozens of embryos from each isolation (about 100 buds). Seeds were obtained from plants regenerated from microsporederived embryos after colchicine treatment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98cfef9f0827a70cc9d3e77ff3c02805 https://doi.org/10.1007/bf00232537 Zobrazit plný text záznamu Full text from SpringerLink