Multifunctional nanoplatform based on star-shaped copolymer for liver cancer targeting therapy

Autor: Xiaowei Zeng, Kebing Chen, Guangzhi He, Yi Zheng, Zhihong Chen, Xianling Gong
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Polymers
Pharmaceutical Science
02 engineering and technology
star-shaped copolymer
030226 pharmacology & pharmacy
Polyethylene Glycols
chemistry.chemical_compound
Mice
0302 clinical medicine
Drug Delivery Systems
Zeta potential
media_common
Chemistry
Liver Neoplasms
General Medicine
Hep G2 Cells
021001 nanoscience & nanotechnology
nanomedicine
targeting drug delivery
PLGA
Liver
Lactates
Female
0210 nano-technology
Liver cancer
Research Article
Drug
media_common.quotation_subject
Mice
Nude
Antineoplastic Agents
cancer nanotechnology
Cholic Acid
macromolecular substances
03 medical and health sciences
Cell Line
Tumor
PEG ratio
medicine
Distribution (pharmacology)
Animals
Humans
Lactic Acid
Particle Size
lcsh:RM1-950
Cholic acid
technology
industry
and agriculture
lactobionic acid
medicine.disease
Lactobionic acid
Drug Liberation
lcsh:Therapeutics. Pharmacology
Cancer research
Nanoparticles
Polyglycolic Acid
Zdroj: Drug Delivery, Vol 26, Iss 1, Pp 595-603 (2019)
Drug Delivery
ISSN: 1521-0464
1071-7544
Popis: With high morbidity and death rates, liver cancer has become one of the most common cancers in the world. But, most chemotherapeutic anticancer drugs have high toxicity as well as low specificity. To improve the treatment modalities and enhance the therapeutic effect of liver cancer, a brand new liver-targeting nanoparticle (NP), Ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic acid (5 F)-loaded cholic acid (CA)-functionalized star-shaped poly (lactic-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-lactobionic acid (LA) (5 F-loaded CA-PLGA-PEG-LA), was developed. The particle size, zeta potential, size distribution, surface morphology, drug loading content, drug encapsulation efficiency and drug release of 5 F-loaded NPs were characterized. Confocal microscopy and flow cytometry showed that the prepared NPs could be internalized by HepG2 cells. Furthermore, the cellular uptake efficiency of coumarin 6-loaded CA-PLGA-PEG-LA NPs was much better in compare with that of CA-PLGA-PEG and CA-PLGA NPs. Moreover, LA-conjugated NPs (CA-PLGA-PEG-LA NPs) enhanced fluorescence of HepG2 cells via ligand-mediated endocytosis. The antitumor effects of 5 F-loaded NPs were evaluated by the MTT assay in vitro and by a xenograft tumor model in vivo, demonstrating that targeted 5 F-loaded CA-PLGA-PEG-LA NPs were significantly superior to free 5 F and 5 F-loaded CA-PLGA-PEG NPs. All the results indicated the 5 F-loaded CA-PLGA-PEG-LA NPs can be employed as a novel potentially targeting drug delivery system for liver cancer therapy.
Databáze: OpenAIRE
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