Localization and Fine Mapping of Antigenic Sites on the Nucleocapsid Protein N of Porcine Reproductive and Respiratory Syndrome Virus with Monoclonal Antibodies
| Autor: | Rob H. Meloen, Langeveld Jan, A. van Essen-Zandbergen, A.P. van Nieuwstadt, J.N.A. Bos-de Ruijter, Johanna Jacoba Maria Meulenberg |
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| Rok vydání: | 1998 |
| Předmět: |
Recombinant Fusion Proteins
Molecular Sequence Data Semliki Forest virus Binding Competitive Peptide Mapping Epitope Virus Cell Line Mice Antibody Specificity Cricetinae Virology Animals Life Science Porcine respiratory and reproductive syndrome virus Amino Acid Sequence Cloning Molecular Peptide sequence Instituut voor Dierhouderij en Diergezondheid chemistry.chemical_classification Binding Sites biology ID-Lelystad Antibodies Monoclonal Nucleocapsid Proteins Porcine reproductive and respiratory syndrome virus biology.organism_classification Molecular biology Amino acid ID Lelystad Epitope mapping Pepscan chemistry ID-Lelystad Instituut voor Dierhouderij en Diergezondheid DNA Viral ID Lelystad Institute for Animal Science and Health Epitope Mapping Institute for Animal Science and Health |
| Zdroj: | Virology, 252, 106-114 Virology 252 (1998) |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1998.9436 |
| Popis: | The purpose of this study was to analyze the antigenic structure of the nucleocapsid protein N of the Lelystad virus isolate of porcine reproductive and respiratory syndrome virus (PRRSV) and to identify antigenic differences between this prototype European isolate and other North American isolates. To do this, we generated a panel of monoclonal antibodies (mAbs) directed against the N protein of Lelystad virus and tested them in competition assays with other N-specific mAbs described previously (Drewet al.,1995; Nelsonet al.,1993; van Nieuwstadtet al.,1996). Four different competition groups of mAbs were identified. Pepscan analysis with solid-phase dodecapeptides was used to identify specific antigenic regions in the N protein that were bound by the mAbs. In this pepscan analysis, we found that the mAb of the first competition group reacted with linear peptides whose core sequences consisted of amino acids 2–12 (site A), the mAbs of the second group reacted with peptides whose core sequences consisted of amino acids 25–30 (site B), and the mAb of the third group reacted with peptides whose core sequences consisted of amino acids 40–46 (site C). However, the fourth group of mAbs binding to an antigenic region, provisionally designated as domain D, reacted very weakly or did not react at all with solid-phase dodecapeptides.To further characterize the structure of the epitopes in domain D, we produced chimeric constructs composed of the N protein sequences of Lelystad virus and another arterivirus lactate dehydrogenase-elevating virus, which was used because its N protein has similarity in amino acid sequence and hydropathicity profile but does not react with our mAbs. When the mAbs specific to domain D were tested for binding to the chimeric N proteins expressed by Semliki Forest virus, we found that the regions between amino acids 51–67 and amino acids 80–90 are involved in the formation or are part of the epitopes in domain D. Therefore, we conclude that the N protein contains four distinct antigenic regions. The epitopes mapped to sites A–C are linear, whereas the epitopes mapped to domain D are more conformation dependent or discontinuous. Sites A and C contain epitopes that are conserved in European but not in North American isolates; site B contains epitopes that are conserved in European and North American isolates; and site D contains epitopes that are either conserved or not conserved in European and North American isolates. The antigenic regions identified here might be important for the development of diagnostic test for PRRSV in particular tests that discriminate between different antigenic types of PRRSV. |
| Databáze: | OpenAIRE |
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