Ex situ evaluation of the composition of protein corona of intravenously injected superparamagnetic nanoparticles in rats

Autor: Heinrich Hofmann, Usawadee Sakulkhu, Farhad Rezaee, Marcel P. de Vries, Lionel Maurizi, Azza Gramoun, Morteza Mahmoudi, Marie-Gabrielle Ollivier Beuzelin, M. M. Motazacker, Jean-Paul Vallée
Přispěvatelé: Laboratoire Interdisciplinaire Carnot de Bourgogne [Dijon] (LICB), Université de Bourgogne (UB)-Université de Technologie de Belfort-Montbeliard (UTBM)-Centre National de la Recherche Scientifique (CNRS), Harvard Medical School [Boston] (HMS), Geneva University Hospital (HUG), Department of Immunology, Powder Technology Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), ACS - Amsterdam Cardiovascular Sciences, Human Genetics
Předmět:
inorganic chemicals
Biodistribution
endocrine system
Materials science
Nanoparticle
COAGULATION
Nanotechnology
Protein Corona
ddc:616.0757
chemistry.chemical_compound
Coated Materials
Biocompatible
In vivo
Dextrans/administration & dosage/chemistry
Materials Testing
Animals
General Materials Science
VITRO
Surface charge
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Magnetite Nanoparticles
health care economics and organizations
IN-VIVO
chemistry.chemical_classification
Coated Materials
Biocompatible/administration & dosage/chemistry
Biomolecule
IRON-OXIDE NANOPARTICLES
technology
industry
and agriculture
Dextrans
Blood Proteins
CANCER
In vitro
Magnetite Nanoparticles/administration & dosage/chemistry
Rats
chemistry
Rats
Inbred Lew
Polyvinyl Alcohol
PHYSICOCHEMICAL PROPERTIES
Injections
Intravenous
CELLS
Biophysics
Female
Polyvinyl Alcohol/chemistry
Adsorption
Iron oxide nanoparticles
Blood Proteins/chemistry/ultrastructure
Protein Binding
Zdroj: Nanoscale
Nanoscale, Royal Society of Chemistry, 2014, 6 (19), pp.11439-11450. ⟨10.1039/C4NR02793K⟩
Nanoscale, Vol. 6, No 19 (2014) pp. 11439-1150
Nanoscale, 6(19), 11439-11450. Royal Society of Chemistry
Nanoscale, 6(19), 11439-11450. ROYAL SOC CHEMISTRY
ISSN: 2040-3364
2040-3372
DOI: 10.1039/C4NR02793K⟩
Popis: International audience; It is now well recognized that the surfaces of nanoparticles (NPs) are coated with biomolecules (e.g., proteins) in a biological medium. Although extensive reports have been published o n the protein corona at the surface of NPs in vitro, there are very few on the in vivo protein corona. The main reason for having very poor information regarding the protein corona in vivo is that separation of NPs from the in vivo environment has not been possible by using available techniques. Knowledge of the in vivo protein corona could lead to better understanding and prediction of the fate of NPs in vivo. Here, by using the unique magnetic properties of superparamagnetic iron oxide NPs (SPIONs), NPs were extracted from rat sera after in vivo interaction with the rat's physiological system. More specifically, the in vivo protein coronas of polyvinyl-alcohol-coated SPIONs with various surface charges are defined. The compositions of the corona at the surface of various SPIONs and their effects on the biodistribution of SPIONs were examined and compared with the corona composition of particles incubated for the same time in rat serum.
Databáze: OpenAIRE
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