The down-regulation of galectin-1 expression is a specific biomarker of arsenic toxicity
| Autor: | Yung Hsi Kao, Ming Chang Chiang, Rong-Nan Huang, Tai Chih Kuo, Yu Ying Chang, Li Ling Chi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Antimony
Sodium arsenite Galectin 1 Stereochemistry Blotting Western chemistry.chemical_element Down-Regulation Tetrazolium Salts Toxicology Transfection KB Cells Arsenic chemistry.chemical_compound Mice RNA interference Nickel Arsenic Poisoning Animals Humans Arsenic trioxide Gene knockdown Arsenic toxicity Spectrophotometry Atomic Cell Cycle General Medicine 3T3 Cells Flow Cytometry Molecular biology carbohydrates (lipids) Thiazoles chemistry Galectin-1 Cancer cell RNA Interference Biomarkers Cadmium |
| Zdroj: | Toxicology letters. 205(1) |
| ISSN: | 1879-3169 |
| Popis: | Galectin-1 (GAL1) is known as a β-galactoside-binding protein that also can bind with arsenic to regulate cell functions. Using RNA interference technique, we investigated the possible mechanism involved in GAL1 modulation of arsenite-inhibited cell survival in 3T3 fibroblast and KB oral cancer cells. GAL1 gene knockdown significantly attenuated sodium arsenite (NaAsO(2)) and arsenic trioxide (As(2)O(3)) inhibition of cell survival. However, GAL1 gene knockdown did not alter the inhibition of cell survival by antimony chloride, cadmium chloride or nickel sulfate. These results suggested the GAL1 selectively affects particular types of heavy metal elements. Flow cytometric analysis indicated GAL1 gene knockdown also suppressed As(III)-stimulated levels of sub-G1 and G2/M growth arrest in both cells. Moreover, atomic absorption spectrophotometric results showed that GAL1 gene knockdown reduced the total arsenic accumulation of both cells after the NaAsO(2) and As(2)O(3) treatment. These results suggested that GAL1 gene knockdown mediates the apoptotic effects of arsenic in 3T3 and KB cells via regulation of the cellular arsenic levels. We propose that down-regulation of GAL1 expression may be a useful and specific biomarker in assessing the toxicity of arsenic exposure. |
| Databáze: | OpenAIRE |
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