Analysis of the porcine APOA2 gene expression in liver, polymorphism identification and association with fatty acid composition traits

Autor: J.A.P. Marchesi, Josep María Folch, Anna Castelló, Anna Puig-Oliveras, Manuel Revilla, Maria Ballester, Jordi Corominas, Ana Isabel Fernández
Přispěvatelé: Ministerio de Economía y Competitividad (España), Universidad Autónoma de Barcelona, Generalitat de Catalunya, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministerio de Educación y Ciencia (España)
Rok vydání: 2016
Předmět:
0301 basic medicine
Candidate gene
Meat
Genotype
Swine
Linoleic acid
Quantitative Trait Loci
Sus scrofa
Mutation
Missense
Gene Expression
Biology
Polymorphism
Single Nucleotide
Allelic differential expression
Sensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]
03 medical and health sciences
chemistry.chemical_compound
Polymorphism (computer science)
Gene expression
Genetics
Animals
Trans-factors
Meat quality
Allele
Promoter Regions
Genetic
Gene
Alleles
Crosses
Genetic
Genetic Association Studies
chemistry.chemical_classification
Cis-regulatory polymorphism
Fatty Acids
0402 animal and dairy science
Pyrosequencing
Fatty acid
Sequence Analysis
DNA
04 agricultural and veterinary sciences
General Medicine
040201 dairy & animal science
Molecular biology
030104 developmental biology
Adipose Tissue
Liver
chemistry
lipids (amino acids
peptides
and proteins)
Animal Science and Zoology
Apolipoprotein A-II
Polyunsaturated fatty acid
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Repositorio de Resultados de Investigación del INIA
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
INIA: Repositorio de Resultados de Investigación del INIA
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Animal Genetics, 47, 5, pp. 552-9
Animal Genetics, 47, 552-9
ISSN: 0268-9146
Popis: APOA2 is a protein implicated in triglyceride, fatty acid and glucose metabolism. In pigs, the APOA2 gene is located on pig chromosome 4 (SSC4) in a QTL region affecting fatty acid composition, fatness and growth traits. In this study, we evaluated APOA2 as a candidate gene for meat quality traits in an Iberian × Landrace backcross population. The APOA2:c.131T>A polymorphism, located in exon 3 of APOA2 and determining a missense mutation, was associated with the percentage of hexadecenoic acid [C16:1(n–9)], linoleic acid [C18:2(n–6)], α-linolenic acid [C18:3(n–3)], dihomo-gamma-linolenic acid [C20:3(n–6)] and polyunsaturated fatty acids (PUFAs) in backfat. Furthermore, this SNP was associated with the global mRNA expression levels of APOA2 in liver and was used as a marker to determine allelic expression imbalance by pyrosequencing. We determined an overexpression of the T allele in heterozygous samples with a mean ratio of 2.8 (T/A), observing a high variability in the allelic expression among individuals. This result suggests that complex regulatory mechanisms, beyond a single polymorphism (e.g. epigenetic effects or multiple cis-acting polymorphisms), may be regulating APOA2 gene expression.
This work was funded by MINECO AGL2011-29821-C02 and AGL2014-56369-C2 grants. M. Ballester is financially supported by a Ramon y Cajal contract (RYC-2013-12573) from the Spanish Ministry of Economy and Competitiveness. A. Puig-Oliveras was funded by a PIF PhD grant from Universitat Autònoma de Barcelona (458-01-1/2011), J. Corominas was funded by a FPI PhD grant from the Spanish Ministry of Education (BES-2009-081223), M. Revilla was funded by a Formació i Contractació de Personal Investigador Novell (FI-DGR) PhD grant from Generalitat de Catalunya (ECO/1639/2013) and J.A.P. Marchesi was funded by a CNPq scholarship from the Brazilian Science Without Borders Program.
Databáze: OpenAIRE
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