Analysis of core region from egg white lysozyme forming amyloid fibrils
| Autor: | Yuhei Tokunaga, Yoshiki Kamada, Kei-ichi Watanabe, Yasushi Sugimoto, Yukako Sakakibara |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Amyloid Biophysics Peptide macromolecular substances Fibril Microscopy Atomic Force Applied Microbiology and Biotechnology Fluorescence Protein Structure Secondary chemistry.chemical_compound Microscopy Electron Transmission Animals Humans tryptophan Amino Acids Molecular Biology lysozyme Ecology Evolution Behavior and Systematics chemistry.chemical_classification Oligopeptide Tryptophan Temperature Cell Biology Hydrogen-Ion Concentration Amino acid Kinetics chemistry Biochemistry core region egg white Muramidase Lysozyme Chickens Oligopeptides Developmental Biology Egg white Research Paper amyloid fibril formation |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| Popis: | Some of the lysozyme mutants in humans cause systemic amyloidosis. Hen egg white lysozyme (HEWL) has been well studied as a model protein of amyloid fibrils formation. We previously identified an amyloid core region consisting of nine amino acids (designated as the K peptide), which is present at 54-62 in HEWL. The K peptide, with tryptophan at its C- terminus, has the ability of self-aggregation. In the present work we focused on its structural properties in relation to the formation of fibrils. The K peptide alone formed definite fibrils having β-sheet structures by incubation of 7 days under acidic conditions at 37°C. A substantial number of fibrils were generated under this pH condition and incubation period. Deletion and substitution of tryptophan in the K peptide resulted in no formation of fibrils. Tryptophan 62 in lysozyme was suggested to be especially crucial to forming amyloid fibrils. We also show that amyloid fibrils formation of the K peptide requires not only tryptophan 62 but also a certain length containing hydrophobic amino acids. A core region is involved in the significant formation of amyloid fibrils of lysozyme. |
| Databáze: | OpenAIRE |
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