Genotypes and genetic characters of Mycobacterium tuberculosis from Myanmar using three typing methods
| Autor: | Mi Mi Htwe, Chulhun L. Chang, Su Mon Win, Hyeyoung Lee, Kim Yeun, Workneh Korma, Wint Wint Nyunt, Phyu Win Ei, Thyn Lei Swe, Wah Wah Aung, Jong-Seok Lee, Si Thu Aung |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male Tuberculosis Adolescent Genotype 030106 microbiology Minisatellite Repeats Myanmar Microbiology Mycobacterium tuberculosis 03 medical and health sciences Young Adult Genetics medicine Humans Typing Molecular Biology Genotyping Ecology Evolution Behavior and Systematics Phylogeny Aged Aged 80 and over Molecular Epidemiology biology Molecular epidemiology Geography Genetic Variation Middle Aged bacterial infections and mycoses biology.organism_classification medicine.disease Molecular Typing 030104 developmental biology Infectious Diseases Female Restriction fragment length polymorphism Rifampicin Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 75 |
| ISSN: | 1567-7257 |
| Popis: | Knowledge on basic characteristics of Mycobacterium tuberculosis (MTB) is helpful to understand the disease epidemiology and support the prediction of clinical outcome of the disease. The aim of this study was to detect the genotypes and genotypic characters of clinical Mycobacterium tuberculosis (MTB) isolates from new and retreatment rifampicin-resistant patients using three different genotyping methods. Mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing was used to determine the diversity of 222 clinical isolates. Spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) typing were also used to investigate the genetic characters of 105 MTB strains. Among the 15 genotypes detected by MIRU-VNTR, Beijing strains were the most prevalent of all strains (54.8%); new cases (40.5%) and retreatment cases (69.4%), followed by EAI strain. Spoligotyping categorized the strains into 11 lineages and 13 orphans whereas 96 different IS6110 patterns were identified using RFLP method. The mode number of IS6110 was 18 and 20. Higher band numbers were found in Beijing genotype (p 0.001). Clustering rates by spoligotyping, MIRU-VNTR and IS6110-RFLP typing were 0.714, 0.004 and 0.085, respectively. Discriminatory powers of spoligotyping, MIRU-VNTR typing and IS6110-RFLP typing were 0.637, 1.000 and 0.997, respectively. Dominant Beijing genotype in both new and retreatment cases denoting that prevailing tuberculosis in Myanmar changed from EAI to Beijing lineage. |
| Databáze: | OpenAIRE |
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