NF-kappaB activation precedes increases in mRNA encoding neurokinin-1 receptor, proinflammatory cytokines, and adhesion molecules in dextran sulfate sodium-induced colitis in rats
| Autor: | Michael J. O'Brien, Chris Andry, A. Brent Fruin, Karen L. Reed, Kelly D. Gonzales, James M. Becker, Arthur F. Stucchi, Adam C. Gower |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Physiology medicine.medical_treatment Molecular Sequence Data Inflammation Substance P Sensitivity and Specificity Proinflammatory cytokine Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Internal medicine medicine Animals RNA Messenger Colitis Receptor Probability Analysis of Variance biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Biopsy Needle Dextran Sulfate Gastroenterology NF-kappa B Receptors Neurokinin-1 medicine.disease Immunohistochemistry Rats Disease Models Animal Cytokine Endocrinology chemistry Myeloperoxidase biology.protein Cytokines Tumor necrosis factor alpha medicine.symptom Inflammation Mediators |
| Zdroj: | Digestive diseases and sciences. 50(12) |
| ISSN: | 0163-2116 |
| Popis: | Nuclear factor kappa B (NF-kappa B) plays a key role in initiating inflammation associated with colitis. A systematic study was conducted in the rat DSS colitis model to determine the temporal relationship between NF-kappa B activation and expression of substance P (SP), neurokinin-1 receptor (NK-1R), proinflammatory cytokines, and adhesion molecules. Rats were given 5% DSS in their water and sacrificed daily for 6 days. Colon tissue was collected for assessment of histological changes, NF-kappa B activation, myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS. NF-kappa B activation increased, biphasically, on Day 1 and again on Days 4-6. The mRNA levels for ICAM-1, CINC-1, IL-1beta, TNFalpha, VCAM-1, and NK-1R rose significantly (P < 0.05) by 2-4 days. Increased iNOS mRNA levels, MPO activity, and mucosal damage occurred on Day 6. These data demonstrate that NF-kappa B activation substantially precedes the onset of physical disease signs and active inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink