Leucine supplementation protects from insulin resistance by regulating adiposity levels
| Autor: | Caroline André, E. Binder, Melissa Elie, llaria Belluomo, Sophie Layé, Samantha Clark, Uberto Pagotto, Gilles Mithieux, Flaminia Fanelli, Agnès Aubert, Marco Mezzullo, Thierry Leste-Lasserre, Francisco Javier Bermúdez-Silva, Daniela Cota, A. Duchampt, Silvana Y. Romero-Zerbo |
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| Přispěvatelé: | Neurocentre Magendie, Physiopathologie de la plasticité neuronale, U862, Institut National de la Santé et de la Recherche Médicale (INSERM), Hospital Carlos Haya, Nutrition et Neurobiologie intégrée (NutriNeuro), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Centre National de la Recherche Scientifique (CNRS), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Marcia B. Aguila, Elke Binder, Francisco J. Bermúdez-Silva, Caroline André, Melissa Elie, Silvana Y. Romero-Zerbo, Thierry Leste-Lasserre, llaria Belluomo, Adeline Duchampt, Samantha Clark, Agnes Aubert, Marco Mezzullo, Flaminia Fanelli, Uberto Pagotto, Sophie Layé, Gilles Mithieux, Daniela Cota, ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Centre National de la Recherche Scientifique (CNRS), [Binder,E, Bermúdez-Silva,FJ, André,C, Elie,M, Romero-Zerbo,SY, Lester-Lasserre,T, Belluomo,I, Clark,S, Cota,D] INSERM, Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, Bordeaux, France. [Binder,E, Belluomo,I, Clark,S] Université de Bordeaux, Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, Bordeaux, France. [Bermúdez-Silva,FJ, Romero-Zerbo,SY] IBIMA-Hospital Carlos Haya, Laboratorio de Investigación, Malaga, Spain. [Duchampt,A, Mithieux,G] INSERM, Lyon, France. [Duchampt,A, Mithieux,G, Cota,D] Université de Lyon, Lyon, France. [Duchampt,A, Mithieux,G] Université Lyon 1, Villeurbanne, France. [Aubert,A, Layé,S] Nutrition et Neurobiologie Intégrée, Université de Bordeaux, Bordeaux, France. INRA, Nutrition et Neurobiologie Intégrée, Bordeaux, France. [Mezzullo,M, Fanelli,F] Endocrinology Unit and Centro di Ricerca Biomedica Applicata, Department of Clinical Medicine, S.Orsola-Malpighi Hospital, Alma Mater University of Bologna, Bologna, Italy., This work was supported by INSERM, Aquitaine Region, Ajinomoto 3ARP research program, ANR-2010-1414-01 and EquipEx OptoPath ANR-10-EQPX-08 (to DC), European Community’s Seventh Framework Programme FP7-People2009-IEF-251494 (DC and EB) and Fondation Recherche Médicale. FJBS is recipient of a research contract from the National System of Health (Instituto de Salud Carlos III, CP07/00283) and of a BAE from Instituto de Salud Carlos III (BA09/90066). |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
food intake Ratones [SDV]Life Sciences [q-bio] medicine.medical_treatment Obesidad Mice Obese tissu adipeux Type 2 diabetes test de tolérance souris Weight Gain Mice 0302 clinical medicine Brown adipose tissue Homeostasis Insulin Uncoupling protein Aminoácidos Adiposity 2. Zero hunger 0303 health sciences Multidisciplinary Diseases::Pathological Conditions Signs and Symptoms::Signs and Symptoms::Body Weight::Overweight::Obesity [Medical Subject Headings] Fatty Acids digestive oral and skin physiology santé humaine Lipids Pérdida de peso 3. Good health [SDV] Life Sciences [q-bio] adiposité obésité Phenotype medicine.anatomical_structure Medicine Leucine leucine Colesterol Oxidation-Reduction Research Article diabète medicine.medical_specialty Science 030209 endocrinology & metabolism Carbohydrate metabolism Biology masse graisseuse Diet High-Fat Alimentación rica en grasa 03 medical and health sciences Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Diet::Diet High-Fat [Medical Subject Headings] Insulin resistance Leptina pcr Internal medicine medicine Animals Diseases::Pathological Conditions Signs and Symptoms::Signs and Symptoms::Body Weight::Body Weight Changes::Weight Loss [Medical Subject Headings] Chemicals and Drugs::Amino Acids Peptides and Proteins::Amino Acids [Medical Subject Headings] baisse de poids Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Peptide Hormones::Adipokines::Leptin [Medical Subject Headings] insuline 030304 developmental biology Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] medicine.disease Chemicals and Drugs::Lipids::Sterols::Cholesterol [Medical Subject Headings] Mice Inbred C57BL Glucose Endocrinology Dietary Supplements Insulin Resistance Energy Metabolism Phenomena and Processes::Physiological Phenomena::Physiological Processes::Homeostasis [Medical Subject Headings] |
| Zdroj: | Plos One 9 (8), 12 p.. (2013) PLoS ONE PLoS ONE, Vol 8, Iss 9, p e74705 (2013) PLoS ONE, Public Library of Science, 2013, 8 (9), 12 p. ⟨10.1371/journal.pone.0074705⟩ PLoS ONE, 2013, 8 (9), 12 p. ⟨10.1371/journal.pone.0074705⟩ |
| ISSN: | 1932-6203 |
| Popis: | International audience; Background Leucine supplementation might have therapeutic potential in preventing diet-induced obesity and improving insulin sensitivity. However, the underlying mechanisms are at present unclear. Additionally, it is unclear whether leucine supplementation might be equally efficacious once obesity has developed. Methodology/Principal Findings Male C57BL/6J mice were fed chow or a high-fat diet (HFD), supplemented or not with leucine for 17 weeks. Another group of HFD-fed mice (HFD-pairfat group) was food restricted in order to reach an adiposity level comparable to that of HFD-Leu mice. Finally, a third group of mice was exposed to HFD for 12 weeks before being chronically supplemented with leucine. Leucine supplementation in HFD-fed mice decreased body weight and fat mass by increasing energy expenditure, fatty acid oxidation and locomotor activity in vivo. The decreased adiposity in HFD-Leu mice was associated with increased expression of uncoupling protein 3 (UCP-3) in the brown adipose tissue, better insulin sensitivity, increased intestinal gluconeogenesis and preservation of islets of Langerhans histomorphology and function. HFD-pairfat mice had a comparable improvement in insulin sensitivity, without changes in islets physiology or intestinal gluconeogenesis. Remarkably, both HFD-Leu and HFD-pairfat mice had decreased hepatic lipid content, which likely helped improve insulin sensitivity. In contrast, when leucine was supplemented to already obese animals, no changes in body weight, body composition or glucose metabolism were observed. Conclusions/Significance These findings suggest that leucine improves insulin sensitivity in HFD-fed mice by primarily decreasing adiposity, rather than directly acting on peripheral target organs. However, beneficial effects of leucine on intestinal gluconeogenesis and islets of Langerhans's physiology might help prevent type 2 diabetes development. Differently, metabolic benefit of leucine supplementation is lacking in already obese animals, a phenomenon possibly related to the extent of the obesity before starting the supplementation. |
| Databáze: | OpenAIRE |
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