Hepatic Fatty Acid Balance and Hepatic Fat Content in Humans With Severe Obesity
| Autor: | Nikki C. Bush, Kelli A Lytle, James M. Swain, Michael L. Kendrick, Kazanna C. Hames, Michael D. Jensen, Nicola Gathaiya, Todd A. Kellogg, Jessica M. Triay |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Very low-density lipoprotein medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism Clinical Biochemistry Bariatric Surgery 030209 endocrinology & metabolism Fatty Acids Nonesterified Lipoproteins VLDL Biochemistry 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Endocrinology Non-alcoholic Fatty Liver Disease Internal medicine Nonalcoholic fatty liver disease medicine Humans Beta oxidation Triglycerides Clinical Research Articles chemistry.chemical_classification Sphingolipids Triglyceride Biochemistry (medical) Fatty liver Fatty Acids Fatty acid Middle Aged medicine.disease Lipid Metabolism Obesity Morbid 030104 developmental biology chemistry Liver Ketone bodies lipids (amino acids peptides and proteins) Female Lipoprotein |
| Zdroj: | J Clin Endocrinol Metab |
| ISSN: | 1945-7197 |
| Popis: | ObjectiveNonalcoholic fatty liver disease can lead to hepatic inflammation/damage. Understanding the physiological mechanisms that contribute to excess hepatic lipid accumulation may help identify effective treatments.DesignWe recruited 25 nondiabetic patients with severe obesity scheduled for bariatric surgery. To evaluate liver export of triglyceride fatty acids, we measured very-low-density lipoprotein (VLDL)–triglyceride secretion rates the day prior to surgery using an infusion of autologous [1-14C]triolein-labeled VLDL particles. Ketone body response to fasting and intrahepatic long-chain acylcarnitine concentrations were used as indices of hepatic fatty acid oxidation. We measured intraoperative hepatic uptake rates of plasma free fatty acids using a continuous infusion of [U-13C]palmitate, combined with a bolus dose of [9,10-3H]palmitate and carefully timed liver biopsies. Total intrahepatic lipids were measured in liver biopsy samples to determine fatty liver status. The hepatic concentrations and enrichment from [U-13C]palmitate in diacylglycerols, sphingolipids, and acyl-carnitines were measured using liquid chromatography/tandem mass spectrometry.ResultsAmong study participants with fatty liver disease, intrahepatic lipid was negatively correlated with VLDL-triglyceride secretion rates (r = −0.92, P = 0.01) but unrelated to hepatic free fatty acid uptake or indices of hepatic fatty acid oxidation. VLDL-triglyceride secretion rates were positively correlated with hepatic concentrations of saturated diacylglycerol (r = 0.46, P = 0.02) and sphingosine-1-phosphate (r = 0.44, P = 0.03).ConclusionWe conclude that in nondiabetic humans with severe obesity, excess intrahepatic lipid is associated with limited export of triglyceride in VLDL particles rather than increased uptake of systemic free fatty acids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|