An alternative and sensitive method based on LCM and Q-PCR for HER2 testing in breast cancer
| Autor: | Meda Rus, Ovidiu Balacescu, Bogdan Fetica, Ioana Berindan-Neagoe, Loredana Balacescu |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Receptor ErbB-2 Chromogenic in situ hybridization Breast Neoplasms Pilot Projects Laser Capture Microdissection Biology Real-Time Polymerase Chain Reaction Polymerase Chain Reaction Sensitivity and Specificity Breast cancer Gene duplication Genetics medicine Humans skin and connective tissue diseases CISH In Situ Hybridization medicine.diagnostic_test General Medicine Gold standard (test) medicine.disease Molecular diagnostics Immunohistochemistry Real-time polymerase chain reaction Oncology Cancer research Female Fluorescence in situ hybridization |
| Zdroj: | Cancer biomarkers : section A of Disease markers. 14(2-3) |
| ISSN: | 1875-8592 |
| Popis: | Nowadays, HER2 testing in breast cancer represents a necessity for both prognostic and therapy. Despite widespread use of immunohistochemistry (IHC) for assessing HER2 status, there are some limitations to identify truly negative or positive HER2 cases. Fluorescence in situ hybridization (FISH) or chromogenic in situ hybridization (CISH) could solve the equivocal HER2 IHC cases but there is no consensus on which is the best method. Consequently, finding a sensitive method for HER2 testing is critical for the management of the disease. In addition, tumor heterogeneity is an important factor which could affect accuracy of molecular diagnostics. Laser capture micro-dissection (LCM) is used to isolate pure cell populations from heterogeneous tumor tissue. The combination between LCM and quantitative polymerase chain reaction (Q-PCR), the gold standard in molecular biology for quantifying gene amplification levels, could define an important tool to improve the molecular diagnostics of HER2 status.In our pilot study we used LCM and Q-PCR to evaluate HER2 gene amplification for invasive breast carcinoma samples. The samples were selected based on HER2 status assessed by IHC and CISH. Our results demonstrated high sensitivity of Q-PCR for assessing HER2 DNA amplification as well as a good concordance between Q-PCR and IHC/ CISH assay. |
| Databáze: | OpenAIRE |
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