Deletion of 55 open reading frames from the termini of vaccinia virus
| Autor: | Stephen W. Davis, Marion E. Perkus, Gerard P. Johnson, Elizabeth K. Norton, Enzo Paoletti, Scott J. Goebel |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Genes
Viral viruses Molecular Sequence Data Vaccinia virus Genome Open Reading Frames Nucleic acid thermodynamics chemistry.chemical_compound Plasmid Tandem repeat Virology Humans Orthopoxvirus ORFS Repeated sequence Repetitive Sequences Nucleic Acid Genetics Base Sequence biology Nucleic Acid Hybridization biology.organism_classification Molecular biology Blotting Southern chemistry DNA Viral Chromosome Deletion Vaccinia Plasmids |
| Zdroj: | Virology. 180:406-410 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(91)90047-f |
| Popis: | Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the left and the right termini of the vaccinia genome. We report here the engineered deletion within a single vaccinia virus of 32.7 kb of DNA (including 38 ORFS) from the left terminus and 14.9 kb of DNA (including 17 ORFS) from the right terminus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|