Endotoxin has acute and chronic effects on the cerebral circulation of fetal sheep
| Autor: | Elaine Maria Stockx, Susan Ying Shan Feng, David James Phillips, Victor Y H Yu, Adrian M. Walker |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Physiology Ischemia Hemodynamics Blood Pressure Cerebral circulation Fetus Oxygen Consumption Pregnancy Physiology (medical) Internal medicine medicine Animals Fetal Death Nitrites Cerebrospinal Fluid Nitrates Sheep business.industry Tumor Necrosis Factor-alpha Oxygen transport Endothelial Cells Hypoxia (medical) medicine.disease Endotoxins Vasodilation medicine.anatomical_structure Endocrinology Cerebral blood flow Anesthesia Cerebrovascular Circulation Data Interpretation Statistical Vascular resistance Cytokines Female medicine.symptom business |
| Zdroj: | American journal of physiology. Regulatory, integrative and comparative physiology. 296(3) |
| ISSN: | 0363-6119 |
| Popis: | We studied the impact of endotoxemia on cerebral blood flow (CBF), cerebral vascular resistance (CVR), and cerebral oxygen transport (O2 transport) in fetal sheep. We hypothesized that endotoxemia impairs CBF regulation and O2 transport, exposing the brain to hypoxic-ischemic injury. Responses to lipopolysaccharide (LPS; 1 μg/kg iv on 3 consecutive days, n = 9) or normal saline ( n = 5) were studied. Of LPS-treated fetuses, five survived and four died; in surviving fetuses, transient cerebral vasoconstriction at 0.5 h (ΔCVR approximately +50%) was followed by vasodilatation maximal at 5–6 h (ΔCVR approximately −50%) when CBF had increased (approximately +60%) despite reduced ABP (approximately −20%). Decreased CVR and increased CBF persisted 24 h post-LPS and the two subsequent LPS infusions. Cerebral O2 transport was sustained, although arterial O2 saturation was reduced ( P < 0.05). Histological evidence of neuronal injury was found in all surviving LPS-treated fetuses; one experienced grade IV intracranial hemorrhage. Bradykinin-induced cerebral vasodilatation (ΔCVR approximately −20%, P < 0.05) was abolished after LPS. Fetuses that died post-LPS ( n = 4) differed from survivors in three respects: CVR did not fall, CBF did not rise, and O2 transport fell progressively. In conclusion, endotoxin disrupts the cerebral circulation in two phases: 1) acute vasoconstriction (1 h) and 2) prolonged vasodilatation despite impaired endothelial dilatation (24 h). In surviving fetuses, LPS causes brain injury despite cerebral O2 transport being maintained by elevated cerebral perfusion; thus sustained O2 transport does not prevent brain injury in endotoxemia. In contrast, cerebral hypoperfusion and reduced O2 transport occur in fetuses destined to die, emphasizing the importance of sustaining O2 transport for survival. |
| Databáze: | OpenAIRE |
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