Technology for cell cycle research with unstressed steady-state cultures

Autor:	Valerie S. LeBleu, Steven R. Gonda, Maureen Thornton, Charles E. Helmstetter
Rok vydání:	2006
Předmět:	Original Paper education.field_of_study Cell division Chemistry Clinical Biochemistry Population Biomedical Engineering Bioengineering Cell Biology Cell cycle medicine.disease Cell biology On cells Leukemia Cell culture medicine Steady state (chemistry) education Culture vessel Biotechnology
Zdroj:	Cytotechnology. 51:149-157
ISSN:	1573-0778 0920-9069
DOI:	10.1007/s10616-006-9024-5
Popis:	A culture system for performing cell cycle analyses on cells in undisturbed steady-state populations was designed and tested. In this system, newborn cells are shed continuously from an immobilized, perfused culture rotating about the horizontal axis. As a result of this arrangement, the number of newborn cells released into the effluent medium each generation is identical to the number of cells residing in the immobilized population, indicating that one of the two new daughter cells is shed at each cell division. Thus, the immobilized cells constitute a continuous, steady-state culture because the concentrations, locations and microenvironments of the cells in the culture vessel do not vary with time. In tests with mouse L1210 lymphocytic leukemia cells, about 10(8) newborn cells were produced per day. This new culture system enables a multiplicity of cell cycle analyses on large numbers of cells assured to be from populations in steady-state growth.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98b755a0cb3d83cbaad5bb7935b5b713 https://doi.org/10.1007/s10616-006-9024-5 Zobrazit plný text záznamu Full text from SpringerLink