Detrimental effects of oxidative stress in bovine oocytes during intracytoplasmic sperm injection (ICSI)
| Autor: | Shiori Ashibe, Yoku Kato, Rui Miyamoto, Yoshikazu Nagao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
DNA Fragmentation medicine.disease_cause Antioxidants Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization Food Animals medicine Animals Sperm Injections Intracytoplasmic Blastocyst Small Animals reproductive and urinary physiology Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species 030219 obstetrics & reproductive medicine urogenital system Equine 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences 040201 dairy & animal science Oxygen Oxidative Stress Pregnancy rate medicine.anatomical_structure chemistry embryonic structures Oocytes DNA fragmentation Cattle Animal Science and Zoology Oxidative stress |
| Zdroj: | Theriogenology. 133:71-78 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2019.04.012 |
| Popis: | Intracytoplasmic sperm injection (ICSI) is an essential technology in animal and human reproduction. However, the developmental competence and pregnancy rate of embryos derived from ICSI are still lower than that from the conventional in vitro fertilization technique. In this report, we focused on reactive oxygen species (ROS) as a potential detrimental factor for ICSI. Experiment 1 was conducted to evaluate the effect of oxidative stress by two different oxygen concentrations (20%: control vs. 5%) in ICSI on the developmental competence (blastocyst rate: day 7, DNA fragmentation rate: day 4) and, ROS concentration and mitochondrial membrane potential of oocytes in ICSI. In the 5% O2 group, the blastocyst rate (29.5%) was higher and DNA fragmentation rate (4.8 ± 1.0%) was lower than those in the control group significantly (12.7% and 18.2 ± 2.4%, respectively, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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