Relations of enzymes inAspergillus repens grown under sodium chloride stress

Autor:	H. S. Chhatpar, Uddhav Kelavkar
Rok vydání:	1993
Předmět:	chemistry.chemical_classification Sodium Glutamate dehydrogenase chemistry.chemical_element Dehydrogenase General Medicine Biology Applied Microbiology and Biotechnology Microbiology Malate dehydrogenase De novo synthesis Enzyme Invertase chemistry Biochemistry Halotolerance
Zdroj:	Current Microbiology. 27:157-162
ISSN:	1432-0991 0343-8651
Popis:	Aspergillus repens, a salt-pan isolate, was halotolerant. When grown for 72 h (log phase) and 144 h (beginning of stationary phase) in a medium containing 2M sodium chloride, the activities of invertase, malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PDH), and glutamate dehydrogenase (GDH) were found to have increased. Control cultures grown in a medium devoid of 2M NaCl failed to show such changes. The activities of MDH, G6PDH, and GDH increased with rising concentrations of Na(+) (as NaCl) when added up to 100MM in vitro. At higher concentrations they decreased. Changes in kinetic constants, Km and Vmax of these enzymes, as well as their de novo synthesis, were found to be some of the responses to NaCl stress-mediated changes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98af95d5d7c2fef0e441ae72de4f0053 https://doi.org/10.1007/bf01576014 Zobrazit plný text záznamu Full text from SpringerLink