Anti-listerial properties of chemical constituents of Eruca sativa (rocket salad): From industrial observation to in vitro activity
| Autor: | Gesine Schmidt, Trond Møretrø, Sileshi Gizachew Wubshet, Solveig Langsrud, Annette Fagerlund, Grethe Iren A. Borge |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Food Handling
Glycobiology Eruca Bacterial growth Pathology and Laboratory Medicine medicine.disease_cause Biochemistry Nutrient Animal Products Tandem Mass Spectrometry Vegetables Medicine and Health Sciences Food science Chromatography High Pressure Liquid 0303 health sciences Multidisciplinary biology Microbiota Pseudomonas Eukaryota Agriculture Nucleosides Genomics Plants Contamination Glycosylamines Bacterial Pathogens Cold Temperature Fruit and Vegetable Juices Medical Microbiology Medicine Pathogens Research Article Meat Listeria Science Microbial Genomics Microbiology 03 medical and health sciences Listeria monocytogenes Genetics medicine Microbial Pathogens Nutrition 030304 developmental biology Bacteria Plant Extracts 030306 microbiology business.industry Organisms Biology and Life Sciences Listeria Monocytogenes biology.organism_classification Diet Seafood Food Brassicaceae Food Microbiology Food processing Microbiome business |
| Zdroj: | PLOS ONE PLoS ONE PLoS ONE, Vol 16, Iss 4, p e0250648 (2021) |
| Popis: | The frequency of foodborne outbreaks epidemiologically associated with Listeria monocytogenes in fresh produce has increased in recent years. Although L. monocytogenes may be transferred from the environment to vegetables during farming, contamination of food products most commonly occurs in food processing facilities, where L. monocytogenes has the ability to establish and persist on processing equipment. The current study was undertaken to collect data on the occurrence of L. monocytogenes and the identity of the endogenous microbiota in a fresh produce processing facility, for which information has remained scarce. L. monocytogenes was not detected in the facility. Experiments simulating conditions in the processing environment were performed, including examination of bacterial growth in nutrients based on vegetables (salad juice) compared to in other types of nutrients (fish, meat). Results showed that the endogenous microbiota (dominated by Pseudomonas) grew well in iceberg lettuce and rocket salad juice at low temperatures, while growth inhibition of L. monocytogenes was observed, particularly in rocket salad juice. The anti-listerial activity in rocket salad juice was retained in a polar chromatographic fraction containing several metabolites. Characterization of this active fraction, using LC-MS/MS, led to identification of 19 compounds including nucleosides and amino acids. Further work is necessary to determine the molecular mechanism responsible for the inhibitory activity of rocket salad constituents. The study nevertheless suggests that the available nutrients, as well as a low temperature (3 °C) and the in-house bacterial flora, may influence the prevalence of L. monocytogenes in fresh produce processing facilities. |
| Databáze: | OpenAIRE |
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