Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection
| Autor: | O. A. Averina, Alexander M. Mazur, Alexey Starshin, Olga O. Grigorieva, Egor Prokhortchouk, Olga A. Dontsova, Oleg A. Permyakov, Petr V. Sergiev |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA End-Joining Repair
Microinjections Zygote QH301-705.5 DNA Single-Stranded homologous recombination Computational biology Biology Genome Catalysis Genome engineering Inorganic Chemistry Mice Genome editing microinjection Animals CRISPR genome editing Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Gene Editing Cas9 Communication Organic Chemistry General Medicine Computer Science Applications Chemistry Models Animal prime editor CRISPR-Cas Systems Homologous recombination Functional genomics knockout mice RNA Guide Kinetoplastida CRISPR Cas9 |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 10221, p 10221 (2021) International Journal of Molecular Sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations. |
| Databáze: | OpenAIRE |
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