Purification and characterization of ubiquitin from mammalian testis

Autor:	J.P. Muh, Pierre Sautiere, Yves Menezo, Maurice Loir, A. Caraty, M. Lanneau
Rok vydání:	1984
Předmět:	Male Gel filtration chromatography Chromosomal Proteins Non-Histone Protein Conformation Size-exclusion chromatography Radioimmunoassay Biophysics Biology Biochemistry Protein structure Ubiquitin Structural Biology Testis Genetics Animals Amino Acid Sequence Amino Acids Ubiquitins Molecular Biology Peptide sequence Mammals chemistry.chemical_classification Mammalian testis Cell Biology Molecular biology Nucleoprotein Amino acid Nucleoproteins chemistry Chromatography Gel biology.protein Oxidation-Reduction Amino acid analysis
Zdroj:	FEBS Letters. 169:199-204
ISSN:	0014-5793
Popis:	Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98aaba81a40a52d2998a139a7843f289 https://doi.org/10.1016/0014-5793(84)80318-x Zobrazit plný text záznamu Plný text